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Modulation by $Zn^{2+}$ of GABA responses in bipolar cells of the mouse retina
| Content Provider | Scilit |
|---|---|
| Author | Kaneda, M. Andrásfalvy, B. Kaneko, A. |
| Copyright Year | 2000 |
| Description | The localization of endogenous $Zn^{2+}$ in the mouse retina was examined histochemically and the inhibitory action of $Zn^{2+}$ on GABA-induced responses was studied in bipolar cells isolated from the mouse retina. Accumulation of endogenous $Zn^{2+}$ was detected in photoreceptors, bipolar, and/or amacrine cells by either the bromopyridylazo-diethylaminophenol method or the dithizone method. Under whole-cell recording conditions, GABA induced a $Cl^{−}$ current in isolated bipolar cells. The current consisted of two components. The first component was inhibited completely by application of 100 μM bicuculline, suggesting that this is a $GABA_{A}$-receptor mediated current. The second component was inhibited completely by 100 μM 3-aminopropyl-(methyl)-phosphinic acid, suggesting that this is a $GABA_{C}$-receptor mediated current. $GABA_{C}$ receptors were present at a higher density on the axon terminal than on dendrites. $Zn^{2+}$ inhibited both $GABA_{A}$ and $GABA_{C}$ receptors. $GABA_{C}$ receptors were more susceptible to $Zn^{2+}$; the $IC_{50}$ for the $GABA_{A}$ receptor was 67.4 μM and that for the $GABA_{C}$ receptor was 1.9 μM. These results suggest that $Zn^{2+}$ modulates the inhibitory interaction between amacrine and bipolar cells, particularly that mediated by the $GABA_{C}$ receptor. |
| Related Links | https://www.cambridge.org/core/services/aop-cambridge-core/content/view/0197A5CDF975AF990CFA76D59836D6DE/S0952523800172098a.pdf/div-class-title-modulation-by-zn-span-class-sup-2-span-of-gaba-responses-in-bipolar-cells-of-the-mouse-retina-div.pdf |
| Ending Page | 281 |
| Page Count | 9 |
| Starting Page | 273 |
| ISSN | 09525238 |
| e-ISSN | 14698714 |
| DOI | 10.1017/s0952523800172098 |
| Journal | Visual neuroscience |
| Issue Number | 2 |
| Volume Number | 17 |
| Language | English |
| Publisher | Cambridge University Press (CUP) |
| Publisher Date | 2000-01-01 |
| Access Restriction | Open |
| Subject Keyword | Visual neuroscience Biochemistry and Molecular Biology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Physiology Sensory Systems |
