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Boerhaaviadiffusa L. attenuates angiotensin II-induced hypertrophy in H9c2 cardiac myoblast cells via modulating oxidative stress and down-regulating NF-κβ and transforming growth factor β1
| Content Provider | Scilit |
|---|---|
| Author | Prathapan, A. Vineetha, V. P. Abhilash, P. A. Raghu, K. G. |
| Copyright Year | 2013 |
| Description | The present study evaluated the antihypertrophic potential of the ethanolic extract of Boerhaavia diffusa (BDE), a well-known edible cardiotonic plant reported in Ayurveda against angiotensin II-induced hypertrophy in H9c2 cardiac myoblast cells. Markers of hypertrophy such as cell size, protein content and the concentrations of atrial natriuretic peptide (ANP) and B-type natriuretic peptide (BNP) were analysed for the confirmation of hypertrophy induction. Angiotensin II (100 nm) caused an increase in cell volume (69·26 (sd 1·21) %), protein content (48·48 (sd 1·64) %), ANP (81·90 (sd 1·22) %) and BNP (108·57 (sd 1·47) %). BDE treatment significantly reduced cell volume, protein content and the concentrations of ANP and BNP (P≤ 0·05) in H9c2 cells. The activity of various antioxidant enzymes and the concentration of reduced glutathione, which was lowered due to hypertrophy, were increased in BDE-treated cells. The BDE treatment also reduced intracellular reactive oxygen species generation, lipid peroxidation and protein carbonyls in cells. In addition, the expression patterns of NF-κβ and transforming growth factor β1 were found to be increased during hypertrophy, and their expressions were reduced on BDE treatment. In vitro chemical assays showed that BDE inhibits angiotensin-converting enzyme and xanthine oxidase in a dose-dependent manner with an estimated 50 % effective concentration $(EC_{50}$) value of 166·12 (sd 2·42) and 60·05 (sd 1·54) μg/ml, respectively. The overall results clearly indicate the therapeutic potential of B. diffusa against cardiac hypertrophy, in addition to its nutritional qualities. |
| Related Links | https://www.cambridge.org/core/services/aop-cambridge-core/content/view/DF77CA617DA7DD15A725308FDEBA6629/S0007114513000561a.pdf/div-class-title-span-class-italic-boerhaavia-span-span-class-italic-diffusa-span-l-attenuates-angiotensin-ii-induced-hypertrophy-in-h9c2-cardiac-myoblast-cells-via-modulating-oxidative-stress-and-down-regulating-nf-and-transforming-growth-factor-1-div.pdf |
| Ending Page | 1210 |
| Page Count | 10 |
| Starting Page | 1201 |
| ISSN | 00071145 |
| e-ISSN | 14752662 |
| DOI | 10.1017/s0007114513000561 |
| Journal | British Journal Of Nutrition |
| Issue Number | 7 |
| Volume Number | 110 |
| Language | English |
| Publisher | Cambridge University Press (CUP) |
| Publisher Date | 2013-04-16 |
| Access Restriction | Open |
| Subject Keyword | British Journal Of Nutrition Nutrition and Dietetics Boerhaaviadiffusa Cardiac Hypertrophy Atrial Natriuretic Peptide Reactive Oxygen Species Transcription Factors |
| Content Type | Text |
| Resource Type | Article |
| Subject | Nutrition and Dietetics Medicine |
