NDLI: In vitro shoot regeneration of Stereospermum suaveolens DC. using cotyledonary node and nodal explants

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In vitro shoot regeneration of Stereospermum suaveolens DC. using cotyledonary node and nodal explants

Content Provider	Scilit
Author	Trivedi, Darshini R Joshi, Aruna G
Copyright Year	2015
Description	The cotyledonary node and nodal explants Stereospermum suaveolens DC. were cultured on Woody Plant medium (WPM) supplemented with individual BAP 2?25 ?M), Kn (2?25 ?M) and TDZ (0.1?2 ?M. Observations revealed that in both cotyledonary node and nodal explants, lower concentrations of cytokinins (BAP/Kn/TDZ) were effective in inducing proliferation response. The axillary bud of both the explants proliferated into single shoots which were further subcultured on fresh medium with respective concentrations for shoot multiplication. It was observed that multiple shoots developed only in presence of Kn (8 ?M) in both the explants but the shoots developed from nodal explants were strong and healthy with average shoot number reaching to 3.8 ± 0.6 and length 5.4 ± 0.4 cm by the end of third passage. Rooting (100%) was achieved in microshoots when transferred to half strength WPM liquid medium supplemented with IBA (2 ?M). The plantlets were transferred to different substrates for acclimatization.Plant Tissue Cult. & Biotech. 24(2): 235-246, 2014 (December
Ending Page	246
Page Count	12
Starting Page	235
ISSN	18173721
e-ISSN	18188745
DOI	10.3329/ptcb.v24i2.23556
Alternate Webpage(s)	https://www.banglajol.info/index.php/PTCB/article/download/23556/16142
Journal	Plant Tissue Culture and Biotechnology
Issue Number	2
Volume Number	24
Language	English
Publisher	Bangladesh Academy of Sciences
Publisher Date	2015-06-02
Access Restriction	Open
Subject Keyword	Shoot Regeneration Cotyledonary Node Stereospermum Suaveolens Suaveolens Dc Using Cotyledonary Nodal Explants
Content Type	Text
Resource Type	Article
Subject	Plant Science Biotechnology

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