NDLI: Seroprevalence of anti-HEV and HEV RNA among volunteer blood donors and patients with Hepatitis B and C in Iran

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Seroprevalence of anti-HEV and HEV RNA among volunteer blood donors and patients with Hepatitis B and C in Iran

Content Provider	Scilit
Author	Keyvani, Hossein Shamabadi, Mahmood Shamsi Najafifard, Saeed Hajibeigi, Bashir Fallahian, Farahnaz Alavian, Seyed-Moayed
Copyright Year	1970
Description	Aim: To assess seroprevalence of antibodies to hepatitis E virus (HEV) in healthy blood donors and hepatitis B, C patients. Methods: 450 subjects consisted of 200 blood donors in Tehran blood transfusion center, 100 subjects with hepatitis C and 150 subjects with hepatitis B infection enrolled in this study. The A549 cell line was grown in mixed medium. Cells were infected with hepatitis E virus that was purified from stool sample of a patient confirmed for hepatitis E infection by reverse transcription-polymerase chain reaction (RT-PCR) method. Supernatant of infected cells was used as positive control in our RT- PCR assay. Results: In a total of 450 subjects, 33 (7.3%) had positive anti-HEV by enzyme-linked immunosorbent assay (ELISA). Anti-HEV was seen in (9/200) 4.5%, (7/100) 7%, and (17/150) 11.3% of healthy blood donors, hepatitis C, and hepatitis B subjects, respectively. Difference between two groups was statistically significance (P = 0.028). Difference between frequency of anti-HEV in hepatitis B in relation to healthy blood donors was significant (P = 0.014). Conclusions: HEV infection is more common in subjects with hepatitis B. Keywords: Hepatitis E virus, Seroprevalence, Transmission, Iran doi: 10.3329/blj.v1i1.2623 Bangladesh Liver Journal Vol.1(1) 2009 p.34-37
Ending Page	37
Page Count	4
Starting Page	34
ISSN	20745117
DOI	10.3329/blj.v1i1.2623
Journal	Bangladesh Liver Journal
Issue Number	1
Volume Number	1
Language	English
Publisher	Bangladesh Academy of Sciences
Publisher Date	1970-01-01
Access Restriction	Open
Subject Keyword	Agricultural Engineering Hepatitis E Virus Blood Donors Anti Hev Polymerase Chain Reaction Blood Transfusion
Content Type	Text
Resource Type	Article

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