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Erythropoietin can promote erythroid progenitor survival by repressing apoptosis through Bcl-XL and Bcl-2
| Content Provider | Scilit |
|---|---|
| Author | Silva, M. Grillot, D. Benito, A. Richard, C. Nunez, G. Fernandez-Luna, Jl |
| Copyright Year | 1996 |
| Description | Erythropoietin (Epo), the hormone that is the principal regulator of red blood cell production, interacts with high-affinity receptors on the surface of erythroid progenitor cells and maintains their survival. Epo has been shown to promote cell viability by repressing apoptosis; however, the molecular mechanism involved is unclear. In the present studies we have examined whether Epo acts as a survival factor through the regulation of the bcl-2 family of apoptosis-regulatory genes. We addressed this issue in HCD-57, a murine erythroid progenitor cell line that requires Epo for proliferation and survival. When HCD-57 cells were cultured in the absence of Epo, Bcl-2 and Bcl-XL but not Bax were downregulated, and the cells underwent apoptotic cell death. HCD-57 cells infected with a retroviral vector encoding human Bcl-XL or Bcl-2 rapidly stopped proliferating but remained viable in the absence of Epo. Furthermore, endogenous levels of bcl-2 and bcl-XL were downregulated after Epo withdrawal in HCD-57 cells that remained viable through ectopic expression of human Bcl-XL, further indicating that Epo specifically maintains the expression of bcl-2 and bcl-XL. We also show that HCD-57 rescued from apoptosis by ectopic expression of Bcl-XL can undergo erythroid differentiation in the absence of Epo, demonstrating that a survival signal but not Epo itself is necessary for erythroid differentiation of HCD-57 progenitor cells. Thus, we propose a model whereby Epo functions as a survival factor by repressing apoptosis through Bcl-XL and Bcl-2 during proliferation and differentiation of erythroid progenitors. |
| Related Links | https://ashpublications.org/blood/article-pdf/88/5/1576/233637/1576.pdf |
| Ending Page | 1582 |
| Page Count | 7 |
| Starting Page | 1576 |
| DOI | 10.1182/blood.v88.5.1576.bloodjournal8851576 |
| Journal | Blood |
| Issue Number | 5 |
| Volume Number | 88 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1996-09-01 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Differentiation Cell Death Apoptosis Survival Epo Erythroid Bcl Viable Cells Were Cultured Journal: Blood (Vol- 83, Issue- 5) |
| Content Type | Text |
| Resource Type | Article |
