NDLI: Key role of glycoprotein Ib/V/IX and von Willebrand factor in platelet activation-dependent fibrin formation at low shear flow

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Key role of glycoprotein Ib/V/IX and von Willebrand factor in platelet activation-dependent fibrin formation at low shear flow

Content Provider	Scilit
Author	Cosemans, Judith M. E. M. Schols, Saskia E. M. Stefanini, Lucia Witt, Susanne De Feijge, Marion A. H. Hamulyák, Karly Deckmyn, Hans Bergmeier, Wolfgang Heemskerk, Johan W. M.
Copyright Year	2011
Description	A microscopic method was developed to study the role of platelets in fibrin formation. Perfusion of adhered platelets with plasma under coagulating conditions at a low shear rate (250−1) resulted in the assembly of a star-like fibrin network at the platelet surface. The focal fibrin formation on platelets was preceded by rises in cytosolic Ca2+, morphologic changes, and phosphatidylserine exposure. Fibrin formation was slightly affected by αIIbβ3 blockage, but it was greatly delayed and reduced by the following: inhibition of thrombin or platelet activation; interference in the binding of von Willebrand factor (VWF) to glycoprotein Ib/V/IX (GpIb-V-IX); plasma or blood from patients with type 1 von Willebrand disease; and plasma from mice deficient in VWF or the extracellular domain of GpIbα. In this process, the GpIb-binding A1 domain of VWF was similarly effective as full-length VWF. Prestimulation of platelets enhanced the formation of fibrin, which was abrogated by blockage of phosphatidylserine. Together, these results show that, in the presence of thrombin and low shear flow, VWF-induced activation of GpIb-V-IX triggers platelet procoagulant activity and anchorage of a star-like fibrin network. This process can be relevant in hemostasis and the manifestation of von Willebrand disease.
Related Links	http://www.bloodjournal.org/content/117/2/651.full.pdf
Ending Page	660
Page Count	10
Starting Page	651
DOI	10.1182/blood-2010-01-262683
Journal	Blood
Issue Number	2
Volume Number	117
Language	English
Publisher	American Society of Hematology
Publisher Date	2011-01-13
Access Restriction	Open
Subject Keyword	Hematology Platelet Star Glycoprotein Von Willebrand Fibrin Formation Low Shear Flow Like Fibrin Journal: Blood (Vol- 60, Issue- 2)
Content Type	Text
Resource Type	Article

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