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Platelet membrane glycoprotein IIb heavy chain forms a complex with glycoprotein IIIa that binds Arg-Gly-Asp peptides
| Content Provider | Scilit |
|---|---|
| Author | Lam, Sc Plow, Ef Ginsberg, Mh |
| Copyright Year | 1989 |
| Description | Platelet membrane GPIIb is comprised of a disulfide-linked heavy chain (GPIIb(H)) and light chain (GPIIb(L)). We have examined the role of the two chains of GPIIb in the maintenance of the GPIIb-IIIa heterodimer and Arg-Gly-Asp (RGD) peptide-binding function. Lysates of surface radioiodinated platelets were treated with 1% 2-mercaptoethanol for 18 hours at 4 degrees C. Reduction of the interchain disulfide in GPIIb was followed by immunoprecipitation with antipeptide antibodies specific for GPIIb(H) or GPIIb(L). In addition to the GPIIb-IIIa complex, a polypeptide of 120 Kd was precipitated by anti-GPIIb(H) and a polypeptide of 23 Kd was precipitated by anti-GPIIb(L) from reduced platelet lysates. To determine whether GPIIb(H) or GPIIb(L) remained complexed with GPIIIa, reduced platelet lysates were immunoprecipitated with AP3, a monoclonal anti-GPIIIa antibody, resulting in the coimmunoprecipitation of GPIIb(H) but not GPIIb(L). Conversely, the monoclonal anti-GPIIb(H) antibody PMI-1 immunoprecipitated GPIIIa with GPIIb(H). Thus GPIIb(H) maintains its association with GPIIIa. Furthermore, the GPIIb(H)-IIIa complex retains its reactivity with AP2, a monoclonal antibody (MoAb) specific for the nondissociated GPIIb-IIIa complex. Affinity chromatography of reduced platelet lysates on immobilized KYGRGDS resulted in binding and specific elution of the GPIIb(H)-IIIa complex. These findings indicate that GPIIb(H) contains sufficient information for maintenance of a complex with GPIIIa and support of the binding of the heterodimer to RGD peptides. |
| Related Links | https://ashpublications.org/blood/article-pdf/73/6/1513/216016/1513.pdf |
| Ending Page | 1518 |
| Page Count | 6 |
| Starting Page | 1513 |
| DOI | 10.1182/blood.v73.6.1513.bloodjournal7361513 |
| Journal | Blood |
| Issue Number | 6 |
| Volume Number | 73 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1989-05-01 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Antibody Function Platelet Glycoprotein Chain Gpiib Gpiiia Lysates Iiia Complex Journal: Blood (Vol- 88, Issue- 6) |
| Content Type | Text |
| Resource Type | Article |
