NDLI: Phenotypic and clonal analysis of T lymphocytes in childhood immune thrombocytopenic purpura

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Phenotypic and clonal analysis of T lymphocytes in childhood immune thrombocytopenic purpura

Content Provider	Scilit
Author	Ware, Re Howard, Ta
Copyright Year	1993
Description	In an attempt to identify and characterize T-lymphocyte immunoregulatory abnormalities in immune thrombocytopenic purpura (ITP), we have performed phenotypic and clonal analysis on peripheral T lymphocytes from 23 children with ITP. Quantitation of lymphocyte subpopulations showed that children with acute ITP had higher numbers of CD45RA+ and lower numbers of CD45RO+ T cells than children with chronic ITP or controls, but these differences may be age related. Analysis of T-cell receptor variable beta gene usage identified 2 boys with chronic ITP and elevated numbers of V beta 8+ T cells. Eight T- cell clones were established (6 CD4+, 4B4+ helper-inducer lines and 2 CD8+ lines) that showed in vitro proliferation against allogeneic platelets. The addition of autologous antigen-presenting cells enhanced the proliferation of six clones, but not for two clones that coexpressed natural killer (NK) markers. Four of seven positive clones also had measurable interleukin (IL)-2 secretion following platelet stimulation, providing further evidence for T-cell reactivity. Our results provide the first evidence that patients with ITP may have platelet-reactive T lymphocytes identifiable at the clonal level, supporting the hypothesis that autoreactive peripheral T lymphocytes may mediate or participate in the pathogenesis of this disorder.
Related Links	https://ashpublications.org/blood/article-pdf/82/7/2137/611607/2137.pdf
Ending Page	2142
Page Count	6
Starting Page	2137
DOI	10.1182/blood.v82.7.2137.2137
Journal	Blood
Issue Number	7
Volume Number	82
Language	English
Publisher	American Society of Hematology
Publisher Date	1993-10-01
Access Restriction	Open
Subject Keyword	Pediatrics and Child Health Children Lymphocytes Proliferation Purpura Itp Antigen Beta Clonal Clones Thrombocytopenic Journal: Blood (Vol- 97, Issue- 7)
Content Type	Text
Resource Type	Article

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