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Dysfunction and infection of freshly isolated blood myeloid and plasmacytoid dendritic cells in patients infected with HIV-1
| Content Provider | Scilit |
|---|---|
| Author | Donaghy, Heather Gazzard, Brian Gotch, Frances Patterson, Steven |
| Copyright Year | 2003 |
| Description | Recently it has been shown that the 2 populations of blood dendritic cells (DCs), termed plasmacytoid (pcDCs) and myeloid (myDCs), are reduced in HIV-1 infection. This study aimed to determine whether these 2 populations are targets for HIV-1 infection and whether their ability to stimulate T-lymphocyte proliferation is affected. Highly purified populations of myDCs and pcDCs were isolated from the blood of antiretroviral treatment–naive patients and assessed for the level of HIV provirus by polymerase chain reaction (PCR). We show that both populations are targets for HIV-1 infection as indicated by the presence of provirus in 12 of 14 pcDC and 13 of 14 myDC samples tested. A proportion of this provirus is integrated in myDCs. The ability of both myDCs and pcDCs from HIV-1–infected patients to stimulate allogeneic T-lymphocyte proliferation in a 6-day mixed leukocyte reaction was severely impaired, but was not mediated by secondary infection of T lymphocytes. Thus, in addition to depletion, both myeloid and plasmacytoid DCs are infected and show impaired functional capacity. These findings suggest that infection, depletion, and dysfunction of dendritic cells may contribute to the immunosuppression associated with HIV-1 disease. |
| Related Links | http://bloodjournal.hematologylibrary.org/content/101/11/4505.full.pdf |
| Ending Page | 4511 |
| Page Count | 7 |
| Starting Page | 4505 |
| DOI | 10.1182/blood-2002-10-3189 |
| Journal | Blood |
| Issue Number | 11 |
| Volume Number | 101 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 2003-06-01 |
| Access Restriction | Open |
| Subject Keyword | Virology Hiv Dendritic Cells Provirus Depletion Plasmacytoid Myeloid Functional Treatment Pcdcs Journal: Blood (Vol- 117, Issue- 11) |
| Content Type | Text |
| Resource Type | Article |
