NDLI: Relative increase in leukemia-specific DNA in peripheral blood plasma from patients with acute myeloid leukemia and myelodysplasia

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Relative increase in leukemia-specific DNA in peripheral blood plasma from patients with acute myeloid leukemia and myelodysplasia

Content Provider	Scilit
Author	Rogers, Anna Joe, Youngson Manshouri, Taghi Dey, Amanda Jilani, Iman Giles, Francis Estey, Elihu Freireich, Emil Keating, Michael Kantarjian, Hagop Albitar, Maher
Copyright Year	2004
Description	Using loss of heterozygosity (LOH) and X-chromosome inactivation, we compared peripheral blood (PB) plasma with bone marrow (BM) cells in detecting genomic abnormalities in patients with acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS). We detected LOH in the PB plasma of all 45 patients who had cytogenetically documented chromosomal abnormalities (5q-, 7-, +8, 17-, or 20-). BM cells from the same patients showed LOH in 89% of patients with MDS and 70% of patients with AML. Posttherapy samples from 16 of these patients demonstrated complete concordance between LOH and cytogenetics in detecting residual disease in 15 samples. Of the 16 samples, 4 showed LOH in plasma with normal BM morphology. Using X-chromosome inactivation, clonality was detectable in 19 (73%) of 26 BM samples, whereas all PB plasma samples showed clonality. These data support the conclusion that PB plasma is enriched by tumor-specific DNA and can replace BM cells for studying genomic abnormalities. (Blood. 2004;103:2799-2801)
Related Links	http://pdfs.semanticscholar.org/b04d/b4f138b0614ce84588d2b89364b32b4a9fbf.pdf
Ending Page	2801
Page Count	3
Starting Page	2799
DOI	10.1182/blood-2003-06-1840
Journal	Blood
Issue Number	7
Volume Number	103
Language	English
Publisher	American Society of Hematology
Publisher Date	2004-04-01
Access Restriction	Open
Subject Keyword	Hematology Research and Experimental Medicine Acute Myeloid Leukemia X Chromosome Inactivation Aml Cytogenetics Blood Plasma Specific Dna Myelodysplastic Syndrome Bone Marrow Journal: Blood (Vol- 99, Issue- 7)
Content Type	Text
Resource Type	Article

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