NDLI: Chronic exposure to retroviral vector encoded MGDF (mpl-ligand) induces lineage-specific growth and differentiation of megakaryocytes in mice

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Chronic exposure to retroviral vector encoded MGDF (mpl-ligand) induces lineage-specific growth and differentiation of megakaryocytes in mice

Content Provider	Scilit
Author	Yan, Xq Lacey, D. Fletcher, F. Hartley, C. McElroy, P. Sun, Y. Xia, M. Mu, S. Saris, C. Hill, D.
Copyright Year	1995
Description	Megakaryocyte growth and development factor (MGDF) has recently been identified as a ligand for the c-mpl receptor. Using retroviral- mediated gene transfer, MGDF has been overexpressed in mice to evaluate the systematic effects due to chronic exposure to this growth factor. MGDF overexpressing mice had more rapid platelet recovery than control mice after transplantation. Following this recovery, the platelet levels continued increasing to fourfold to eightfold above normal baseline levels and remained elevated (five-fold above control mice) in these animals, which are alive and well at more than 4 months posttransplantation. Increased megakaryocyte numbers were detected in a number of organs in these mice including bone marrow, spleen, liver, and lymph nodes. Prolonged overexpression of MGDF led to decreased marrow hematopoiesis, especially erythropoiesis, with a shift to extramedullary hematopoiesis in the spleen and liver. All the MGDF overexpressing mice analyzed to date developed myelofibrosis and osteosclerosis, possibly induced by megakaryocyte and platelet produced cytokines. No significant effect on other hematopoietic lineages was seen in the MGDF overexpressing mice, showing that the stimulatory effect of MGDF in vivo is restricted to the megakaryocyte lineage.
Related Links	https://ashpublications.org/blood/article-pdf/86/11/4025/616455/4025.pdf
Ending Page	4033
Page Count	9
Starting Page	4025
DOI	10.1182/blood.v86.11.4025.bloodjournal86114025
Journal	Blood
Issue Number	11
Volume Number	86
Language	English
Publisher	American Society of Hematology
Publisher Date	1995-12-01
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Research and Experimental Medicine Hematopoietic Bone Marrow Hematopoiesis Mpl Megakaryocyte Retroviral Overexpressing Mice Mgdf Overexpressing Journal: Blood (Vol- 116, Issue- 11)
Content Type	Text
Resource Type	Article

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