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Expression of CD41 marks the initiation of definitive hematopoiesis in the mouse embryo
| Content Provider | Scilit |
|---|---|
| Author | Mikkola, Hanna K. A. Fujiwara, Yuko Schlaeger, Thorsten M. Traver, David Orkin, Stuart H. |
| Copyright Year | 2003 |
| Description | Murine hematopoietic stem cells (HSCs) originate from mesoderm in a process that requires the transcription factor SCL/Tal1. To define steps in the commitment to blood cell fate, we compared wild-type and SCL−/− embryonic stem cell differentiation in vitro and identified CD41 (GpIIb) as the earliest surface marker missing from SCL−/− embryoid bodies (EBs). Culture of fluorescence-activated cell sorter (FACS) purified cells from EBs showed that definitive hematopoietic progenitors were highly enriched in the CD41+ fraction, whereas endothelial cells developed from CD41− cells. In the mouse embryo, expression of CD41 was detected in yolk sac blood islands and in fetal liver. In yolk sac and EBs, the panhematopoietic marker CD45 appeared in a subpopulation of CD41+ cells. However, multilineage hematopoietic colonies developed not only from CD45+CD41+ cells but also from CD45−CD41+ cells, suggesting that CD41 rather than CD45 marks the definitive culture colony-forming unit (CFU-C) at the embryonic stage. In contrast, fetal liver CFU-C was CD45+, and only a subfraction expressed CD41, demonstrating down-regulation of CD41 by the fetal liver stage. In yolk sac and EBs, CD41 was coexpressed with embryonic HSC markers c-kit and CD34. Sorting for CD41 and c-kit expression resulted in enrichment of definitive hematopoietic progenitors. Furthermore, the CD41+c-kit+ population was missing from runx1/AML1−/− EBs that lack definitive hematopoiesis. These results suggest that the expression of CD41, a candidate target gene of SCL/Tal1, and c-kit define the divergence of definitive hematopoiesis from endothelial cells during development. Although CD41 is commonly referred to as megakaryocyte–platelet integrin in adult hematopoiesis, these results implicate a wider role for CD41 during murine ontogeny. |
| Related Links | http://www.bloodjournal.org/content/bloodjournal/101/2/508.full.pdf |
| Ending Page | 516 |
| Page Count | 9 |
| Starting Page | 508 |
| DOI | 10.1182/blood-2002-06-1699 |
| Journal | Blood |
| Issue Number | 2 |
| Volume Number | 101 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 2003-01-15 |
| Access Restriction | Open |
| Subject Keyword | Developmental Biology Embryonic Stem Cell Transcription Factor Hsc Cd41 Cd45 Mouse Embryo Scl/tal1 Definitive Hematopoietic Journal: Blood (Vol- 59, Issue- 2) |
| Content Type | Text |
| Resource Type | Article |
