NDLI: Effect of interferon on colony formation in culture by blast cell progenitors in acute myeloblastic leukemia

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Effect of interferon on colony formation in culture by blast cell progenitors in acute myeloblastic leukemia

Content Provider	Scilit
Author	Taetle, R. Buick, Rn McCulloch, Ea
Copyright Year	1980
Description	The effect of purified human fibroblast interferon on primary and secondary colony formation by blast progenitors from the peripheral blood of patients with acute myelogenous leukemia was examined. Interferon inhibited blast progenitors and normal granulocyte/macrophage progenitors (CFU-C) in a dose-dependent manner. The magnitude of this effect on blast progenitors and CFU was similar. Interferon also inhibited secondary plating of blast progenitors (self- renewal). This effect was in marked contrast to the effect of adriamycin, which reduced primary plating efficiency of blast progenitors but did not affect self-renewal. Inhibition of blast progenitor proliferation by interferon was markedly reduced when interferon was added after 24 hr of culture and was absent when added after 72 hr. Inhibition of self-renewal was observed even when interferon was added at 72 hr. We conclude that interferon inhibits both primary proliferation and self-renewal of blast progenitors and that this effect is not due to reduction in the number of primary colonies. These experiments provide an example of how cell culture techniques may be used to test antitumor agents for effects on important cellular events other than general cytotoxicity.
Related Links	https://ashpublications.org/blood/article-pdf/56/3/549/208329/549.pdf
Ending Page	552
Page Count	4
Starting Page	549
DOI	10.1182/blood.v56.3.549.bloodjournal563549
Journal	Blood
Issue Number	3
Volume Number	56
Language	English
Publisher	American Society of Hematology
Publisher Date	1980-09-01
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Hematology Interferon Cell Culture Leukemia Colony Plating Cfu Blast Progenitors Journal: Blood (Vol- 65, Issue- 3)
Content Type	Text
Resource Type	Article

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