NDLI: Isolation and Characterization of Transformed Human T-Cell Lines Infected by Epstein-Barr Virus

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Isolation and Characterization of Transformed Human T-Cell Lines Infected by Epstein-Barr Virus

Content Provider	Scilit
Author	Groux, Hervé Cottrez, Françoise Montpellier, Claire Quatannens, Brigitte Coll, Jean Stehelin, Dominique Auriault, Claude
Copyright Year	1997
Description	Epstein-Barr virus (EBV) is a human lymphotropic virus whose main targets have traditionally been described as B lymphocytes and epithelial cells. Here we report the isolation and characterization of largely monoclonal transformed human T-cell lines infected by EBV. The transformed T cells expressed CD2, CD3, and either CD4 or CD8 surface molecules and more generally displayed the phenotype of naive T cells with a complete and clonal rearrangement of the T-cell receptor. None of the cell lines expressed B cells, natural killer, or myeloid antigens or had immunoglobulins genes rearrangement. They grew in the absence of growth factor; however, they all secreted interleukin-2 after mitogenic activation. Polymerase chain reaction (PCR) analysis showed the presence of EBV DNA in all these cell lines. Moreover, Southern blot analysis of one of these cell lines shows the presence of circular episomic EBV DNA, and by Northern blot or reverse transcriptase-PCR analysis, only the expression of Epstein-Barr nuclear antigen-1 (EBNA-1) and latent membrane protein-1 (LMP-1) genes was detected. Finally, the complete transformed phenotype of this T-cell line was shown by its injection into nude or recombination activating gene 2 (RAG2)-deficient mice that led to the formation of solid tumors.
Related Links	https://ashpublications.org/blood/article-pdf/89/12/4521/755960/4521.pdf
Ending Page	4530
Page Count	10
Starting Page	4521
DOI	10.1182/blood.v89.12.4521
Journal	Blood
Issue Number	12
Volume Number	89
Language	English
Publisher	American Society of Hematology
Publisher Date	1997-06-15
Access Restriction	Open
Subject Keyword	Virology Pcr Virus Rearrangement Cell Lines Antigen Ebv Epstein Barr Blot Journal: Blood (Vol- 120, Issue- 12)
Content Type	Text
Resource Type	Article

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