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β2-glycoprotein-1 autoantibodies from patients with antiphospholipid syndrome are sufficient to potentiate arterial thrombus formation in a mouse model
| Content Provider | Scilit |
|---|---|
| Author | Arad, Ariela Proulle, Valerie Furie, Richard A. Furie, Barbara C. Furie, Bruce |
| Copyright Year | 2011 |
| Description | Antiphospholipid syndrome is characterized by thrombosis, recurrent fetal loss, and the presence of the lupus anticoagulant, anticardiolipin antibodies, or anti–β2-glycoprotein-1 (anti–β2-GP1) antibodies. Although anti–β2-GP1 antibodies have been documented as a biomarker for diagnosis of antiphospholipid syndrome, their direct role in the pathogenesis of thrombosis is unknown. We have demonstrated using intravital microscopy that anti–β2-GP1 autoantibodies purified from the sera of patients with antiphospholipid syndrome complicated by thrombosis greatly amplify thrombus size after laser-induced vessel wall injury in live mice. Anti–β2-GP1 autoantibodies from 3 patients with antiphospholipid syndrome were affinity-purified using human β2-GP1 bound to agarose. The effects of purified anti–β2-GP1 IgG autoantibodies, of anti–β2-GP1–depleted IgG, and of IgG from normal human sera on thrombus formation were measured in mice after arterial injury in the cremaster muscle. Before injury, purified anti–β2-GP1 IgG autoantibodies, anti–β2-GP1 antibody–depleted IgG, or IgG from normal human sera were infused. Increasing amounts of purified anti–β2-GP1 autoantibodies increased thrombus size in a dose-dependent manner, whereas neither anti–β2-GP1 antibody-depleted IgG nor IgG from normal serum affected thrombus size. These results indicate that anti–β2-GP1 IgG autoantibodies in antiphospholipid syndrome patient sera are not only a marker of antiphospholipid syndrome but are directly involved in the pathogenesis of thrombosis. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3069681/pdf |
| Ending Page | 3459 |
| Page Count | 7 |
| Starting Page | 3453 |
| DOI | 10.1182/blood-2010-08-300715 |
| Journal | Blood |
| Issue Number | 12 |
| Volume Number | 117 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 2011-03-24 |
| Access Restriction | Open |
| Subject Keyword | Research and Experimental Medicine Autoantibodies Glycoprotein Thrombus Antiphospholipid Syndrome Mice Biomarker Pathogenesis Anti Gp1 Journal: Blood (Vol- 121, Issue- 12) |
| Content Type | Text |
| Resource Type | Article |
