NDLI: A Histochemical Procedure for Localizing and Evaluating Leukocyte Alkaline Phosphatase Activity in Smears of Blood and Marrow

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A Histochemical Procedure for Localizing and Evaluating Leukocyte Alkaline Phosphatase Activity in Smears of Blood and Marrow

Content Provider	Scilit
Author	Kaplow, Leonard S.
Copyright Year	1955
Description	A rapid and simple method is described for demonstrating the presence of alkaline phosphatase activity in cells of blood and bone marrow. Smears were fixed with 10 per cent formalin in methanol at 0 ± 5 C. for 30 seconds and stained by the azo dye procedure, using propanediol buffer at a pH of 9.6. A "scoring" technic is outlined, which permits the comparison of alkaline phosphatase activities of blood leukocytes of different individuals. Alkaline phosphatase activity was found only in neutrophilic granulocytes and was localized exclusively in the cytoplasm of these cells. In normal adults, 43 to 95 per cent (mean of 78%) of all neutrophils were unstained. Staining was up to ten times more intense in smears from patients with a variety of pathologic disorders.
Related Links	https://ashpublications.org/blood/article-pdf/10/10/1023/747941/1023.pdf
Ending Page	1029
Page Count	7
Starting Page	1023
DOI	10.1182/blood.v10.10.1023.1023
Journal	Blood
Issue Number	10
Volume Number	10
Language	English
Publisher	American Society of Hematology
Publisher Date	1955-10-01
Access Restriction	Open
Subject Keyword	Pathology Bone Marrow Adults Alkaline Phosphatase Activity Neutrophilic Smears Localizing Journal: Blood (Vol- 111, Issue- 10)
Content Type	Text
Resource Type	Article

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