Acute promyelocytic leukemia: clinical relevance of two major PML-RAR alpha isoforms and detection of minimal residual disease by retrotranscriptase/polymerase chain reaction to predict relapse

Content Provider	Scilit
Author	Huang, W. Sun, Gl Li, Xs Cao, Q. Lu, Y. Jang, Gs Zhang, Fq Chai, Jr Wang, Zy Waxman, S.
Copyright Year	1993
Description	Recent data have shown that the PML-RAR alpha fusion gene resulting from translocation t(15;17) is a highly reliable molecular marker of acute promyelocytic leukemia (APL). In this study performed on 97 Chinese patients with APL, the retrotranscriptase/polymerase chain reaction (RT/PCR) was used to evaluate the clinical relevance of the long (L) or short (S) PML-RAR alpha fusion mRNA isoforms and to study minimal residual disease during clinical remission (CR). There were more early deaths during the all-trans retinoic acid (ATRA) induction treatment and more relapses within 2 years of CR in the S-type (6 of 19 cases) than in the L-type group (2 of 33 cases) (P < .025). Among 12 cases analyzed before and after the ATRA-induced CR, 9 cases (75%) showed positive RT/PCR, whereas only 3 cases showed a negative result, justifying the need for chemotherapy after ATRA-induced CR. Eleven of 62 APL patients in CR, after ATRA-induced CR and chemotherapy consolidation (follow-up, from 3 to 72 months), showed positive RT/PCR. Five of them relapsed within 1 to 6 months after the positive test; one converted to negative after further chemotherapy; and 5 remained in CR status without further PCR data. However, the latter 5 cases all received further intensive consolidation therapy after the PCR positivity. These results show that a positive RT/PCR of PML-RAR alpha is a sensitive predictor of relapse in APL.
Related Links	https://ashpublications.org/blood/article-pdf/82/4/1264/612006/1264.pdf
Ending Page	1269
Page Count	6
Starting Page	1264
DOI	10.1182/blood.v82.4.1264.1264
Journal	Blood
Issue Number	4
Volume Number	82
Language	English
Publisher	American Society of Hematology
Publisher Date	1993-08-15
Access Restriction	Open
Subject Keyword	Hematology Medical Laboratory Technology Treatment Chemotherapy Relapse Apl Rt/pcr Pml Rar Rar Alpha Journal: Blood (Vol- 77, Issue- 4)
Content Type	Text
Resource Type	Article