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Sialylated O-glycans and L-selectin sequentially mediate myeloid cell rolling in vivo
| Content Provider | Scilit |
|---|---|
| Author | Ley, K. Zakrzewicz, A. Hanski, C. Stoolman, Lm Kansas, Gs |
| Copyright Year | 1995 |
| Description | Leukocyte rolling precedes firm adhesion and emigration in inflammatory cell recruitment. Both P-selectin, an endothelial lectin that binds to sialylated O-glycans containing sialyl-Lewisx (sLex) on the granulocyte surface, and leukocyte L-selectin have been shown to mediate leukocyte rolling in vivo. Here, we investigate rolling of isolated human neutrophils (PMN), HL-60 promyelocytes, and an L-selectin-transfected cell line (300.19-L) during trauma-induced inflammation in rat mesenteric venules. HL-60 cells, which express no L-selectin but abundant sLex, rolled effectively immediately after abdominal surgery. HL-60 cell rolling was almost completely abolished by pretreatment with sialidase or monoclonal antibody (MoAb) AM-3 recognizing sLex, and was reduced by about 80% by O-sialoglycoprotein-endopeptidase (OSGP). By contrast, 300.19-L cells rolled poorly immediately after surgery but rolled well between 40 and 120 minutes after surgery. Their rolling was completely inhibited by the blocking L-selectin MoAb LAM1–3, but not by a binding control MoAb. PMN express both L-selectin and clustered, sialylated glycoproteins including P-selectin glycoprotein ligand-1 (PSGL-1). PMN showed effective rolling at all times, which was abolished by sialidase or MoAb AM-3 pretreatment during the first 30 minutes after surgery, but not later, when PMN rolling was largely L-selectin-dependent. We conclude that in trauma-induced inflammation, a two-step mechanism accounts for most of myeloid cell rolling, which initially requires O-glycans and subsequently depends on L-selectin function. |
| Related Links | https://ashpublications.org/blood/article-pdf/85/12/3727/614721/3727.pdf |
| Ending Page | 3735 |
| Page Count | 9 |
| Starting Page | 3727 |
| DOI | 10.1182/blood.v85.12.3727.bloodjournal85123727 |
| Journal | Blood |
| Issue Number | 12 |
| Volume Number | 85 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1995-06-15 |
| Access Restriction | Open |
| Subject Keyword | Research and Experimental Medicine Antibody Function Glycoproteins Leukocyte Pmn Selectin Sialylated Cell Rolling Journal: Blood (Vol- 119, Issue- 12) |
| Content Type | Text |
| Resource Type | Article |
