NDLI: A comparison between activated protein C and des-1-41-light chain- activated protein C in reactions with type 1 plasminogen activator inhibitor

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A comparison between activated protein C and des-1-41-light chain- activated protein C in reactions with type 1 plasminogen activator inhibitor

Content Provider	Scilit
Author	Gladson, Cl Schleef, Rr Binder, Br Loskutoff, Dj Griffin, Jh
Copyright Year	1989
Description	This study investigates the role of the gamma-carboxyglutamic acid (gla) containing domain of activated protein C in interactions with both platelet-derived and purified type 1 plasminogen activator inhibitor (PAI-1). The activity of human platelet PAI-1 was neutralized to the same extent by bovine activated protein C and bovine des-1–41- light chain-activated protein C. Both forms of activated protein C formed SDS-stable, divalent-cation independent complexes with platelet PAI-1, as demonstrated by immunoblotting using antibodies directed to either protein C or PAI-1. Since activated protein C neutralized PAI-1, the potential inhibition of the enzyme by PAI-1 was studied. Purified PAI-1 inhibited the amidolytic activity of bovine-activated protein C and bovine des-1–41-light chain-activated protein C with a k2 of 2.85 X 10(4) M-1 sec-1 for both proteins. These data suggest that the gla domain of activated protein C is not required for neutralization of PAI- 1 activity, for complex formation with PAI-1, or for inhibition of the amidolytic activity of activated protein C by PAI-1.
Related Links	https://ashpublications.org/blood/article-pdf/74/1/173/602303/173.pdf
Ending Page	181
Page Count	9
Starting Page	173
DOI	10.1182/blood.v74.1.173.173
Journal	Blood
Issue Number	1
Volume Number	74
Language	English
Publisher	American Society of Hematology
Publisher Date	1989-07-01
Access Restriction	Open
Subject Keyword	Food Science and Technology Immunoblotting Des Gla Light Chain Bovine Activated Domain of Activated Chain Activated Protein Journal: Blood (Vol- 5, Issue- 1)
Content Type	Text
Resource Type	Article

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