Megakaryocyte colony formation from human bone marrow precursors

Content Provider	Scilit
Author	Vainchenker, W. Bouguet, J. Guichard, J. Breton-Gorius, J.
Copyright Year	1979
Description	We report the growth in plasma clot culture of megakaryocyte colonies from adult bone marrow cells with the use of four different sources of erythropoietin (Ep) as the stimulating factor. A major proportion of the megakaryocyte colonies (75%) were pure, while the others were mixed, involving erythroblasts and megakaryocytes. Ultrastructural studies have shown that the maturation of megakaryocytes was essentially normal and that platelet shedding occurred. Megakaryocyte colony formation required a large number of plated cells (greater than 3 X 10(5)/ml). In the absence of erythropoietin, rare spontaneous megakaryocyte colonies could be observed, while no erythroid colonies were present. However, erythropoietin induced a fivefold increase in the total number of colonies. These data suggest that erythropoietin is involved in the differentiation of human megakaryocytes, but that it does not act alone, since another factor related to the number of seeded cells appears essential for the formation of human megakaryocyte colonies.
Related Links	https://ashpublications.org/blood/article-pdf/54/4/940/206808/940.pdf
Ending Page	945
Page Count	6
Starting Page	940
DOI	10.1182/blood.v54.4.940.bloodjournal544940
Journal	Blood
Issue Number	4
Volume Number	54
Language	English
Publisher	American Society of Hematology
Publisher Date	1979-10-01
Access Restriction	Open
Subject Keyword	Hematology Differentiation Bone Marrow Adult Erythropoietin Formation Megakaryocyte Colonies Human Megakaryocyte Journal: Blood (Vol- 74, Issue- 4)
Content Type	Text
Resource Type	Article