BALB/3T3 fibroblast-conditioned medium attracts cultured mast cells derived from W/W but not from mi/mi mutant mice, both of which are deficient in mast cells

Content Provider	Scilit
Author	Jippo-Kanemoto, T. Adachi, S. Ebi, Y. Matsuda, H. Kasugai, T. Nishikawa, S. Kitamura, Y.
Copyright Year	1992
Description	Proliferation of murine mast cells is induced by both T-cell-derived and fibroblast-derived growth factors. Because the most potent T-cell- derived mast cell growth factor, interleukin-3, promotes the migration of mast cells, we investigated whether fibroblast-derived growth factors had the chemoattractive activity as well. Conditioned medium (CM) of BALB/3T3 fibroblasts induced the migration of cultured mast cells (CMC) derived from normal (+/+) mice. BALB/3T3-CM contained the mast cell growth factor (MGF)/stem cell factor (SCF)/kit ligand (KL), which is the ligand for the receptor encoded by the W (c-kit) gene. CMC derived from the spleen of W/W mice lack the extracellular domain of the W (c-kit) receptor, and W/W CMC did not proliferate in response to BALB/3T3-CM. However, W/W CMC did migrate normally toward BALB/3T3-CM and, moreover, the antibody to the extracellular domain of the W (c- kit) receptor did not inhibit the chemoattractive activity of +/+ CMC toward BALB/3T3-CM. These results indicated that MGF/SCF/KL itself did not represent the major chemoattractive activity. On the other hand, BALB/3T3-CM induced neither proliferation nor migration of CMC derived from mi/mi mice. Both W/W and mi/mi mice are deficient in mast cells, but the present results suggest that the mechanism of the abnormality is different between W/W and mi/mi mice.
Related Links	https://ashpublications.org/blood/article-pdf/80/8/1933/225495/1933.pdf
Ending Page	1939
Page Count	7
Starting Page	1933
DOI	10.1182/blood.v80.8.1933.bloodjournal8081933
Journal	Blood
Issue Number	8
Volume Number	80
Language	English
Publisher	American Society of Hematology
Publisher Date	1992-10-15
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Antibody Migration Proliferation Cmc Fibroblast Mast Cells W/w Balb/3t3 Mi/mi Journal: Blood (Vol- 102, Issue- 8)
Content Type	Text
Resource Type	Article