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Reproduction of transfusion-related acute lung injury in an ex vivo lung model [see comments]
| Content Provider | Scilit |
|---|---|
| Author | Seeger, W. Schneider, U. Kreusler, B. Witzleben, E. Von Walmrath, D. Grimminger, F. Neppert, J. |
| Copyright Year | 1990 |
| Description | Leukoagglutinins are implicated in transfusion-related acute lung injury (TRALI). In the present study, severe lung vascular leakage was reproduced by application of a leukoagglutinating antibody of anti-5b specificity in an ex vivo lung model. The antibody originated from a multiparous donor-plasma, observed to cause noncardiogenic edema during transfusion therapy. Heated full plasma (anti-5b-titer 1/128) or purified immunoglobulin G fraction was used for the studies. Ex vivo isolated rabbit lungs were perfused with albumin buffer, and human granulocytes (PMN) were admixed to the recirculating perfusate. In presence of anti-5b antibody plus 5b-positive PMN plus rabbit plasma as complement-source, severe lung edema occurred after a latent period of 3 to 6 hours. Pulmonary artery pressure was only transiently and moderately increased, and the leakage reaction could be traced back to a several-fold increase in lung vascular permeability. In contrast, no vascular leakage was noted in lungs perfused in the absence of anti-5b antibody, PMN, or rabbit plasma. Moreover, no permeability increase occurred on use of 5b-negative PMN. This reproduction of TRALI in an ex vivo lung model corroborates the role of leukoagglutinating antibodies in initiating PMN-dependent respiratory distress and suggests a contribution of concomitant complement activation. |
| Related Links | https://ashpublications.org/blood/article-pdf/76/7/1438/603169/1438.pdf |
| Ending Page | 1444 |
| Page Count | 7 |
| Starting Page | 1438 |
| DOI | 10.1182/blood.v76.7.1438.1438 |
| Journal | Blood |
| Issue Number | 7 |
| Volume Number | 76 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1990-10-01 |
| Access Restriction | Open |
| Subject Keyword | Hematology Model Reproduction Vascular Leakage Severe Lung Trali 5b Antibody Lungs Perfused Journal: Blood (Vol- 94, Issue- 7) |
| Content Type | Text |
| Resource Type | Article |
