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Lethal graft-versus-host disease: modification with allogeneic cultured donor cells
| Content Provider | Scilit |
|---|---|
| Author | Mauch, P. Lipton, Jm Hamilton, B. Obbagy, J. Kudisch, M. Nathan, D. Hellman, S. |
| Copyright Year | 1984 |
| Description | The use of the bone marrow culture technique was studied as a means to prepare donor marrow for bone marrow transplantation to avoid lethal graft-versus-host disease (GVHD). Preliminary experiments demonstrated the rapid loss of theta-positive cells in such cultures, so that theta- positive cells were not detected after 6 days. Initial experiments in C3H/HeJ (H-2k, Hbbd) recipients prepared with 900 rad demonstrated improved survival when 3-day cultured C57BL/6 (H-2b, Hbbs) donor cells were used in place of hind limb marrow for transplantation. However, hemoglobin typing of recipient animals revealed only short-term donor engraftment, with competitive repopulation of recipient marrow occurring. Subsequent experiments were done in 1,200-rad prepared recipients, with long-term donor engraftment demonstrated. The majority of 1,200-rad prepared animals receiving cultured allogeneic cells died of GVHD, but animals receiving 28-day cultured cells had an improved 90- day survival and a delay in GVHD development over animals receiving hind limb marrow or marrow from shorter times in culture. In addition, animals receiving anti-theta-treated, 3-day nonadherent cells had an improved survival (44%) over animals receiving anti-theta-treated hind limb marrow (20%). These experiments demonstrate modest benefit for the use of cultured cells in bone marrow transplantation across major H-2 histocompatibility complex differences. |
| Related Links | https://ashpublications.org/blood/article-pdf/63/5/1112/213092/1112.pdf |
| Ending Page | 1119 |
| Page Count | 8 |
| Starting Page | 1112 |
| DOI | 10.1182/blood.v63.5.1112.bloodjournal6351112 |
| Journal | Blood |
| Issue Number | 5 |
| Volume Number | 63 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1984-05-01 |
| Access Restriction | Open |
| Subject Keyword | Hematology Bone Marrow Theta Cultured Cells Improved Survival Donor Cells Cultured Allogeneic Day Cultured Experiments Demonstrate Journal: Blood (Vol- 81, Issue- 5) |
| Content Type | Text |
| Resource Type | Article |
