Separation of erythroid progenitor cells in mouse bone marrow by isokinetic-gradient sedimentation

Content Provider	Scilit
Author	Misiti, J. Spivak, Jl
Copyright Year	1979
Description	Isokinetic-gradient sedimentation employing a shallow linear gradient of Ficoll in tissue culture medium was used to isolate erythroid progenitor cells (CFU-e) from mouse bone marrow. Following gradient sedimentation, 34% of the total nucleated cells and 48% of the CFU-e applied to the gradient were recovered, and three distinct modal populations of CFU-e could be distinguished. The slowest-migrating population did not require exposure to exogenous erythropoietin in order to form erythroid colonies in vitro. The other two modal populations of CFU-e required exposure to exogenous erythropoietin for differentiation. One of these, constituting 64% of the hormone- dependent CFU-e recovered, migrated with the bulk of the marrow cells, whereas the other migrated ahead of the bulk of the marrow cells. This latter population, which contained 34% of the CFU-e, was recovered with 11% of the marrow cells, representing a twofold to threefold enrichment. BFU-e migrated more slowly than the erythropoietin- dependent CFU-e. Resedimentation studies suggested that the two erythropoietin-dependent CFU-e populations were distinct modal populations. When cells from the fastest-migrating population of erythropoietin-dependent CFU-e were cocultured with unseparated marrow cells, a further twofold to threefold enhancement of erythroid colony formation was obtained. Comparison of isokinetic-gradient sedimentation with discontinuous and continuous albumin density-gradient sedimentation revealed that isokinetic-gradient sedimentation was a more efficient method than the former and a more rapid method than the latter for isolating CFU-e from mouse bone marrow.
Related Links	https://ashpublications.org/blood/article-pdf/54/1/105/206763/105.pdf
Ending Page	116
Page Count	12
Starting Page	105
DOI	10.1182/blood.v54.1.105.bloodjournal541105
Journal	Blood
Issue Number	1
Volume Number	54
Language	English
Publisher	American Society of Hematology
Publisher Date	1979-07-01
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Bone Marrow Mouse Cfu Erythroid Colonies Migrating Population Distinct Modal Isokinetic Gradient Sedimentation Journal: Blood (Vol- 3, Issue- 1)
Content Type	Text
Resource Type	Article