Loading...
Please wait, while we are loading the content...
Similar Documents
Induction of NK activity in large granular lymphocyte leukemia: activation with anti-CD3 monoclonal antibody and interleukin 2
| Content Provider | Scilit |
|---|---|
| Author | Loughran, Tp Jr Draves, Ke Starkebaum, G. Kidd, P. Clark, Ea |
| Copyright Year | 1987 |
| Description | Large granular lymphocyte (LGL) leukemia is a rare disease characterized by clonal expansion of LGL associated with chronic neutropenia, multiple auto-antibodies, and occasionally polyarthritis. We studied cell surface antigen expression and functional activity of leukemic LGL from ten such patients. Using two-color flow cytometric analysis, we found that leukemic LGL from all ten patients expressed the CD3 and HNK-1 markers, while cells from only four patients expressed IgG Fc receptors (FcR). The LGL leukemic cells had little or no NK activity (defined as MHC-nonrestricted cytotoxicity against K562 target cells); however, NK activity could be induced in leukemic LGL by in vitro treatment with as little as 0.05 microgram/mL of anti-CD3 monoclonal antibody. Cell sorting experiments demonstrated that NK activity was induced in CD3+ leukemic LGL (either CD3+, HNK-1+ or CD3+, FcR+) with anti-CD3 monoclonal antibody but not in normal CD3+, FcR- T cells. Treatment with purified interleukin 2 (IL 2) also caused direct activation of some CD3+ leukemic LGL. Despite induction with anti-CD3 MAb or IL 2, activated leukemic LGL did not proliferate or express high density IL 2 receptors detectable by cell sorter analysis. Treatment with alpha interferon had minimal effect on NK activity of LGL leukemic cells. These results suggest that leukemic LGL may provide a useful model for examining the signals required for LGL maturation and activation. |
| Related Links | https://ashpublications.org/blood/article-pdf/69/1/72/35798/72.pdf |
| Ending Page | 78 |
| Page Count | 7 |
| Starting Page | 72 |
| DOI | 10.1182/blood.v69.1.72.bloodjournal69172 |
| Journal | Blood |
| Issue Number | 1 |
| Volume Number | 69 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1987-01-01 |
| Access Restriction | Open |
| Subject Keyword | Hematology Research and Experimental Medicine Antibody Treatment Induction Antigen Nk Activity Anti Cd3 Activity of Lgl Leukemic Journal: Blood (Vol- 4, Issue- 1) |
| Content Type | Text |
| Resource Type | Article |
