NDLI: Regulation of early human hematopoietic (BFU-E and CFU-GEMM) progenitor cells in vitro by interleukin 1-induced fibroblast-conditioned medium

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Regulation of early human hematopoietic (BFU-E and CFU-GEMM) progenitor cells in vitro by interleukin 1-induced fibroblast-conditioned medium

Content Provider	Scilit
Author	Zucali, Jr Broxmeyer, He Dinarello, Ca Gross, Ma Weiner, Rs
Copyright Year	1987
Description	Stimulators of human erythroid burst-forming units (BFU-E) and multipotential colony-forming cells (CFU-GEMM) can be produced by a number of different cell types. A product of human peripheral blood monocytes, interleukin 1 (IL-1), was evaluated for its ability to stimulate fibroblast cultures to produce stimulators of human bone marrow BFU-E and CFU-GEMM colony formation. BFU-E and CFU-GEMM colony formation was evaluated using low-density, nonadherent low-density, and T lymphocyte-depleted nonadherent low-density human bone marrow cells cultured in the presence of a source of pure human erythropoietin. Both human monocyte conditioned medium (MCM) and human recombinant IL-1 (hrIL-1) induced fibroblasts to produce stimulators of BFU-E and CFU- GEMM in a dose-dependent fashion with maximal colony formation occurring when fibroblasts were stimulated by 25% MCM or 140 ng/mL ROO6B hrIL-1, or 1.25 to 5 ng/mL ROO6T hrIL-1. Preincubation of MCM and hrIL-1 with an antibody to IL-1 inactivated the ability of MCM and hrIL- 1 to induce the release of erythroid and multipotential colony stimulating activity from fibroblasts. These results suggest that monocyte-derived IL-1 is involved in regulating the production of humoral stimulators of early human hematopoietic progenitors.
Related Links	https://ashpublications.org/blood/article-pdf/69/1/33/35846/33.pdf
Ending Page	37
Page Count	5
Starting Page	33
DOI	10.1182/blood.v69.1.33.bloodjournal69133
Journal	Blood
Issue Number	1
Volume Number	69
Language	English
Publisher	American Society of Hematology
Publisher Date	1987-01-01
Access Restriction	Open
Subject Keyword	Cell Tissue Engineering Hematopoietic Bone Marrow Fibroblasts Colony Monocyte Cfu Gemm Cells Cultured Stimulators Bfu Journal: Blood (Vol- 4, Issue- 1)
Content Type	Text
Resource Type	Article

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