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Sequence Analysis of the Epstein-Barr Virus (EBV) Latent Membrane Protein-1 Gene and Promoter Region: Identification of Four Variants Among Wild-Type EBV Isolates
| Content Provider | Scilit |
|---|---|
| Author | Sandvej, Kristian Gratama, Jan W. Munch, Mette Zhou, Xiao-Ge Bolhuis, Reinder L. H. Andresen, Brage Storstein Gregersen, Niels Hamilton-Dutoit, Stephen |
| Copyright Year | 1997 |
| Description | Sequence variations in the Epstein-Barr virus (EBV) encoded latent membrane protein-1 (LMP-1) gene have been described in a Chinese nasopharyngeal carcinoma-derived isolate (CAO), and in viral isolates from various EBV-associated tumors. It has been suggested that these genetic changes, which include loss of a Xho I restriction site (position 169425) and a C-terminal 30-base pair (bp) deletion (position 168287-168256), define EBV genotypes associated with increased tumorigenicity or with disease among particular geographic populations. To determine the frequency of LMP-1 variations in European wild-type virus isolates, we sequenced the LMP1 promoter and gene in EBV from lymphoblastoid cell lines from healthy carriers and patients without EBV-associated disease. Sequence changes were often present, and defined at least four main groups of viral isolates, which we designate Groups A through D. The widespread prevalence of LMP-1 sequence variations, particularly the Xho I polymorphism and the 30-bp deletion, indicate that they cannot be used as simple markers for oncogenic viruses related to particular forms of EBV-associated tumor. Several of the structural changes detected occur, however, at sites where they may affect transcription, translation, or function of LMP-1. Future in vitro studies should aim to establish the functional importance of variations at these sites. |
| Related Links | https://ashpublications.org/blood/article-pdf/90/1/323/1038228/323.pdf |
| Ending Page | 330 |
| Page Count | 8 |
| Starting Page | 323 |
| DOI | 10.1182/blood.v90.1.323 |
| Journal | Blood |
| Issue Number | 1 |
| Volume Number | 90 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1997-07-01 |
| Access Restriction | Open |
| Subject Keyword | Virology Protein Virus Functional Isolates Ebv Lmp Structural Epstein Latent Barr Journal: Blood (Vol- 11, Issue- 1) |
| Content Type | Text |
| Resource Type | Article |
