NDLI: Analysis of phorbol ester stimulated human megakaryocyte development

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Analysis of phorbol ester stimulated human megakaryocyte development

Content Provider	Scilit
Author	Roth, Bj Sledge, Gw Jr Straneva, Je Brandt, J. Goheen, M. Hoffman, R.
Copyright Year	1988
Description	Megakaryocytes are relatively rare components of human bone marrow, making the study of their maturation difficult. Phorbol esters can act as differentiating agents in a number of cell systems including murine megakaryocytes. We report the effects of phorbol esters on the previously described long-term human megakaryocytic leukemia cell culture, EST-IU. While two nontransforming phorbols fail to affect these cells, the transforming phorbol 12-O-tetradecanoylphorbol-13- acetate (TPA) induces a phenotype with characteristics of more mature megakaryocytes in a dose-related manner. This phenotype includes an increased adherence to untreated plastic or glass, polyploidization, an increase in cell size, and increased expression of both platelet glycoproteins and factor VIII-related antigen. Two-color flow cytometric analysis allowed simultaneous determinations of DNA content and the expression of surface membrane antigens or alpha-granule constituents, providing evidence that nuclear, membrane, and cytoplasmic maturation occur in parallel in this cellular system. TPA- induced maturation of EST-IU cells provides an important new cellular model for the further study of human megakaryocyte development.
Related Links	https://ashpublications.org/blood/article-pdf/72/1/202/215463/202.pdf
Ending Page	207
Page Count	6
Starting Page	202
DOI	10.1182/blood.v72.1.202.bloodjournal721202
Journal	Blood
Issue Number	1
Volume Number	72
Language	English
Publisher	American Society of Hematology
Publisher Date	1988-07-01
Access Restriction	Open
Subject Keyword	Hematology Bone Marrow Glycoproteins Model Cell Culture Antigen Maturation Plastic Megakaryocytes Phorbol Differentiating Journal: Blood (Vol- 4, Issue- 1)
Content Type	Text
Resource Type	Article

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