NDLI: Cytokine production by primary bone marrow megakaryocytes

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Cytokine production by primary bone marrow megakaryocytes

Content Provider	Scilit
Author	Jiang, S. Levine, Jd Fu, Y. Deng, B. London, R. Groopman, Je Avraham, Hava
Copyright Year	1994
Description	Primary human bone marrow megakaryocytes were studied for their ability to express and release cytokines potentially relevant to their proliferation and/or differentiation. The purity of the bone marrow megakaryocytes was assessed by morphologic and immunocytochemical criteria. Unstimulated marrow megakaryocytes constitutively expressed genes for interleukin-1 beta (IL-1 beta), IL-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), and tumor necrosis factor-alpha (TNF-alpha), by the polymerase chain reaction (PCR) and Northern blot analysis. At the protein level, megakaryocytes secreted significant amounts of IL-1 beta (53.6 +/- 3.6 pg/mL), IL-6 (57.6 +/- 15.6 pg/mL), and GM-CSF (24 +/- 4 pg/mL) but not TNF-alpha. Exposure of human marrow megakaryocytes to IL-1 beta increased the levels of IL-6 (87.3 +/- 2.3 pg/mL) detected in the culture supernatants. Transforming growth factor- beta was also able to stimulate IL-6, IL-1 beta, and GM-CSF secretion, but was less potent than stimulation with phorbol-12-myristate-13- acetate (PMA). The secreted cytokines acted additively to maintain and increase the number of colony-forming unit-megakaryocytes colonies (approximately 35%). These studies demonstrate the production of multiple cytokines by isolated human bone marrow megakaryocytes constitutively or stimulated in vitro. The capacity of human megakaryocytes to synthesize several cytokines known to modulate hematopoietic cells supports the concept that there may be an autocrine mechanism operative in the regulation of megakaryocytopoiesis.
Related Links	https://ashpublications.org/blood/article-pdf/84/12/4151/613621/4151.pdf
Ending Page	4156
Page Count	6
Starting Page	4151
DOI	10.1182/blood.v84.12.4151.bloodjournal84124151
Journal	Blood
Issue Number	12
Volume Number	84
Language	English
Publisher	American Society of Hematology
Publisher Date	1994-12-15
Access Restriction	Open
Subject Keyword	Hematology Differentiation Protein Hematopoietic Bone Marrow Beta Alpha Expressed Genes Pg/ml Marrow Megakaryocytes Journal: Blood (Vol- 119, Issue- 12)
Content Type	Text
Resource Type	Article

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