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Role of c-kit ligand in the expansion of human hematopoietic progenitor cells
| Content Provider | Scilit |
|---|---|
| Author | Brandt, J. Briddell, Ra Srour, Ef Leemhuis, Tb Hoffman, R. |
| Copyright Year | 1992 |
| Description | To test the hypothesis that the c-kit ligand plays an important role in the regulation of early events occurring during human hematopoiesis, we determined the effect of a recombinant form of c-kit ligand, termed mast cell growth factor (MGF), on the high-proliferative potential colony-forming cell (HPP-CFC) and the cell responsible for initiating long-term hematopoiesis in vitro (LTBMIC). MGF alone did not promote HPP-CFC colony formation by CD34+ DR- CD15- marrow cells, but synergistically augmented the ability of a combination of granulocyte- monocyte colony-stimulating factor (GM-CSF) interleukin (IL)-3 and a recombinant GM-CSF/IL-3 fusion protein (FP) to promote the formation of HPP-CFC-derived colonies. MGF had a similarly profound effect on in vitro long-term hematopoiesis. Repeated additions of IL-3, GM-CSF, or FP alone to CD34+ DR- CD15- marrow cells in a stromal cell-free culture system increased cell numbers 10(3)-fold by day 56 of long-term bone marrow culture (LTBMC), while combinations of MGF with IL-3 or FP yielded 10(4)- and 10(5)-fold expansion of cell numbers. Expansion of the number of assayable colony-forming unit-granulocyte-monocyte (CFU- GM) generated during LTBMC was also markedly enhanced when MGF was added in combination with IL-3 or FP. In addition, MGF, IL-3, and FP individually led to a twofold to threefold increase in HPP-CFC numbers after 14 to 21 days of LTBMC. Furthermore, the effects of these cytokines on HPP-CFC expansion during LTBMC were additive. Throughout the LTBMC, cells receiving MGF possessed a higher cloning efficiency than those receiving IL-3, GM-CSF, or FP alone. These data indicate that the c-kit ligand synergistically interacts with a number of cytokines to directly augment the proliferative capacity of primitive human hematopoietic progenitor cells. |
| Related Links | https://ashpublications.org/blood/article-pdf/79/3/634/223353/634.pdf |
| Ending Page | 641 |
| Page Count | 8 |
| Starting Page | 634 |
| DOI | 10.1182/blood.v79.3.634.bloodjournal793634 |
| Journal | Blood |
| Issue Number | 3 |
| Volume Number | 79 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1992-02-01 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Bone Marrow Kit Mgf Hematopoietic Progenitor Cells Colony Forming Granulocyte Monocyte Human Hematopoietic Progenitor Journal: Blood (Vol- 68, Issue- 3) |
| Content Type | Text |
| Resource Type | Article |
