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Bone marrow stromal cells induce myeloid and lymphoid development of the sorted hematopoietic stem cells in vitro
| Content Provider | Scilit |
|---|---|
| Author | Okuyama, R. Koguma, M. Yanai, N. Obinata, M. |
| Copyright Year | 1995 |
| Description | Regulation of development of hematopoietic stem cells was examined by culturing Lin- c-Kit+ Sca1+ stem cells sorted from bone marrow (BM) cells by fluorescence-activated cell sorting on a layer of TBR59, a BM stromal cell line established from simian virus 40 T-antigen gene transgenic mice. The sorted stem cells did not show self-renewal, but two waves (at 7 and 13 days) of a cobblestone formation were induced by the stromal cell layer. The cobblestones were formed by finite cell division (eight divisions on average) of sorted Lin-c-Kit+ Sca1+ stem cells, and divided cells were still immature. The c-Kithigh stem cell population was induced to form the first wave of cobblestone formation committed to myeloid lineage, whereas c-Kitlow population was induced to form the second wave of this formation committed to lymphoid lineage. Both cobblestone formations require c-Kit function, but very late activation antigen-4-vascular cell adhesion molecule-1 interaction plays different parts in the two lineages. |
| Related Links | https://ashpublications.org/blood/article-pdf/86/7/2590/622264/2590.pdf |
| Ending Page | 2597 |
| Page Count | 8 |
| Starting Page | 2590 |
| DOI | 10.1182/blood.v86.7.2590.2590 |
| Journal | Blood |
| Issue Number | 7 |
| Volume Number | 86 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1995-10-01 |
| Access Restriction | Open |
| Subject Keyword | Research and Experimental Medicine Hematopoietic Function Bone Marrow Stem Antigen Division Myeloid Lymphoid Sorted Journal: Blood (Vol- 97, Issue- 7) |
| Content Type | Text |
| Resource Type | Article |
