Long-term human hematopoiesis in SCID-hu mice bearing transplanted fragments of adult bone and bone marrow cells

Content Provider	Scilit
Author	Heike, Y. Ohira, T. Takahashi, M. Saijo, N.
Copyright Year	1995
Description	An attempt was made to establish an SCID-hu murine model of long-term human hematopoiesis by coimplantation of a bone fragment and bone marrow (BM) cells from an adult human. The SCID-hu mice were treated with a cytokine mixture (recombinant human stem cell factor, interleukin-3, granulocyte/macrophage colony-stimulating factor, and granulocyte colony-stimulating factor) for 4 months and were then maintained for further 8 months under cytokine-free conditions. In the peripheral blood, spleen, and implanted bone fragments in the SCID-hu mice that had received both a bone fragment and BM cells, human CD59+ cells were detected 1 year after transplantation; however, they were not detectable in SCID-hu mice that had received either a bone fragment or BM cells only. Thus, implantation of both a bone fragment and BM cells appears to provide a model of long-term adult human hematopoiesis in SCID mice.
Related Links	https://ashpublications.org/blood/article-pdf/86/2/524/616846/524.pdf
Ending Page	530
Page Count	7
Starting Page	524
DOI	10.1182/blood.v86.2.524.bloodjournal862524
Journal	Blood
Issue Number	2
Volume Number	86
Language	English
Publisher	American Society of Hematology
Publisher Date	1995-07-15
Access Restriction	Open
Subject Keyword	Hematology Bone Marrow Model Transplantation Adult Scid Hu Mice Journal: Blood (Vol- 57, Issue- 2)
Content Type	Text
Resource Type	Article