NDLI: Expression of c-jun during macrophage differentiation of HL-60 cells

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Expression of c-jun during macrophage differentiation of HL-60 cells

Content Provider	Scilit
Author	Gaynor, R. Simon, K. Koeffler, P.
Copyright Year	1991
Description	Cellular transcription factors are important in the regulation of cellular genes. Recent studies have indicated that a class of cellular genes known as early response genes are important in the control of cellular growth properties. Two of these genes, c-jun and c-fos, play an important role in the control of cellular differentiation. Because the acute myelogenous leukemia cell line, HL-60, is capable of differentiating to either macrophages or granulocytes, it provides a good model to understand differential gene expression. To determine if the modulation of c-jun was important in the differentiation of HL-60 cells to either macrophages or granulocytes, expression of c-jun mRNA was determined by Northern analysis at various times following treatment with a variety of differentiating agents, including 12- tetradeconyl-phorbol 13-acetate (TPA), retinoic acid (RA), dimethyl sulfoxide (DMSO), or 1,25 dihydroxyvitamin D3 [1,25 (OH)2 D3]. Both TPA and 1,25(OH)2D3, which induce HL-60 cells to differentiate to macrophages, resulted in marked increases in c-jun mRNA; while RA and DMSO, which induce HL-60 cells to differentiate to granulocytes, did not greatly alter c-jun mRNA expression. HL-60 cell lines resistant to macrophage differentiation after exposure to either 1,25(OH)2D3 or TPA did not result in increases in c-jun mRNA. These results suggest that elevation of c-jun mRNA in HL-60 cells correlated temporally with differentiation to macrophages. Thus, c-jun may be a critical cellular transcription factor involved in macrophage differentiation.
Related Links	https://ashpublications.org/blood/article-pdf/77/12/2618/221108/2618.pdf
Ending Page	2623
Page Count	6
Starting Page	2618
DOI	10.1182/blood.v77.12.2618.bloodjournal77122618
Journal	Blood
Issue Number	12
Volume Number	77
Language	English
Publisher	American Society of Hematology
Publisher Date	1991-06-15
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Transcription Factor Gene Expression Macrophages Dmso Jun Macrophage Differentiation Journal: Blood (Vol- 118, Issue- 12)
Content Type	Text
Resource Type	Article

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