Stem cell factor and interleukin-7 synergize to enhance early myelopoiesis in vitro

Content Provider	Scilit
Author	Fahlman, C. Blomhoff, Hk Veiby, Op Mcniece, Ik Jacobsen, Se
Copyright Year	1994
Description	Interleukin-7 (IL-7) has been shown to be a critical factor in murine lymphoid development. It stimulates pre-B cells to divide in the absence of stroma cells and it is an important growth regulator of immature and mature T cells. IL-7 has been shown to synergize with stem cell factor (SCF) to provide a potent growth stimulus for pre-B cells. However, the combined effects of IL-7 and SCF on murine primitive hematopoietic cells in vitro have not been established. In the present study, the effects of recombinant rat (rr) SCF and recombinant human (rh) IL-7 on primitive murine bone marrow progenitors (Lin-Sca1+) were investigated in single-cell cloning experiments. rhIL-7 alone had no proliferative effect on Lin-Sca1+ cells, but in a dose-dependent manner directly enhanced rrSCF-induced colony formation, with an average increase in colony numbers of 2.7-fold. Interestingly, the cells formed in response to SCF and IL-7 were predominantly mature granulocytes. Thus, SCF and IL-7 synergize to stimulate early myelopoiesis in vitro.
Related Links	https://ashpublications.org/blood/article-pdf/84/5/1450/232387/1450.pdf
Ending Page	1456
Page Count	7
Starting Page	1450
DOI	10.1182/blood.v84.5.1450.bloodjournal8451450
Journal	Blood
Issue Number	5
Volume Number	84
Language	English
Publisher	American Society of Hematology
Publisher Date	1994-09-01
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Research and Experimental Medicine Hematopoietic Bone Marrow Stem Lin Colony Recombinant Scf Sca1 Primitive Synergize Journal: Blood (Vol- 83, Issue- 5)
Content Type	Text
Resource Type	Article