NDLI: Human Hematopoietic Progenitors Express Erythropoietin

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Human Hematopoietic Progenitors Express Erythropoietin

Content Provider	Scilit
Author	Stopka, T. Zivny, J. H. Stopkova, P. Prchal, J. F. Prchal, J. T.
Copyright Year	1998
Description	Erythropoietin (EPO) is a factor essential for erythroid cell proliferation, differentiation, and survival. The production of EPO by the kidneys in response to hypoxia and anemia is well documented. To determine whether EPO is also produced by hematopoietic cells, we analyzed the expression of EPO in normal human hematopoietic progenitors and in their progeny. Undifferentiated CD34+lin− hematopoietic progenitors do not have detectable EPO mRNA. Differentiating CD34+ cells that are stimulated with recombinant human EPO in serum-free liquid cultures express both EPO and EPO receptor (EPOR). Because CD34+ cells represent a heterogeneous cell population, we analyzed individual burst-forming units–erythroid (BFU-E) and nonerythroid colony-forming unit–granulocyte-macrophage colonies for EPO mRNA. Only BFU-E colonies were positive for EPO mRNA. Lysates from pooled BFU-E colonies stained positively for EPO by immunoblotting. To further confirm the intrinsic nature of erythroid EPO, we replaced extrinsic EPO in erythroid colony cultures with EPO-mimicking peptide (EMP). We show EPO expression in the EMP-stimulated BFU-Es at both mRNA and protein levels. Stimulation of bone marrow mononuclear cells (BMMCs) with EMP upregulated EPO expression. Furthermore, we found EPO and EPOR mRNAs as well as EPO protein in K562 cells, a human erythroleukemia cell line. Stimulation of K562 cells with EMP upregulated EPO expression. We suggest that EPO of erythroid origin may have a role in the regulation of erythropoiesis.
Related Links	https://ashpublications.org/blood/article-pdf/91/10/3766/237772/3766.pdf
Ending Page	3772
Page Count	7
Starting Page	3766
DOI	10.1182/blood.v91.10.3766.3766_3766_3772
Journal	Blood
Issue Number	10
Volume Number	91
Language	English
Publisher	American Society of Hematology
Publisher Date	1998-05-15
Access Restriction	Open
Subject Keyword	Urology and Nephrology Differentiation Hematopoietic Bone Marrow Immunoblotting Colonies Cd34 Erythroid Epor Epo Expression Journal: Blood (Vol- 113, Issue- 10)
Content Type	Text
Resource Type	Article

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