NDLI: Induction of erythropoietic colonies in a human chronic myelogenous leukemia cell line

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Induction of erythropoietic colonies in a human chronic myelogenous leukemia cell line

Content Provider	Scilit
Author	Hoffman, R. Murnane, Mj Benz, Ej Jr Prohaska, R. Floyd, V. Dainiak, N. Forget, Bg Furthmayr, H.
Copyright Year	1979
Description	The ability of cells derived from the K562 cell line to generate erythropoietic colonies was studied. The K562 cell line was derived from a patient with chronic myelogenous leukemia 8 yr ago by Lozzio and Lozzio. Rare benzidine-positive colonies formed when these cells were cloned in plasma clots (3 +/- 1/10(4) cells), and their number was not substantially increased by the addition of erythropoietin (9.5 +/- 1/10(4) cells). Sodium butyrate was capable of markedly enhancing the number of benzidine-positive colonies (19.5 +/- 1/10(4) cells) formed, while the combination of sodium butyrate plus erythropoietin exerted a synergistic effect on erythropoietic colony formation (57 +/- 4/10(4) cells). The K562 cell line is a long-term culture system that contains human erythropoietic stem cells. This cell line should be useful in future studies on the cellular and molecular events associated with human erythroid cell differentiation.
Related Links	https://ashpublications.org/blood/article-pdf/54/5/1182/206991/1182.pdf
Ending Page	1187
Page Count	6
Starting Page	1182
DOI	10.1182/blood.v54.5.1182.bloodjournal5451182
Journal	Blood
Issue Number	5
Volume Number	54
Language	English
Publisher	American Society of Hematology
Publisher Date	1979-11-01
Access Restriction	Open
Subject Keyword	Hematology Sodium Erythropoietic Colonies Cell Line K562 Cell Lozzio Butyrate Erythropoietin Differentiation Journal: Blood (Vol- 80, Issue- 5)
Content Type	Text
Resource Type	Article

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