NDLI: BCL-W is dispensable for the sustained survival of select Burkitt lymphoma and diffuse large B-cell lymphoma cell lines.

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BCL-W is dispensable for the sustained survival of select Burkitt lymphoma and diffuse large B-cell lymphoma cell lines.

Content Provider	Scilit
Author	Diepstraten, Sarah T. Chang, Catherine Tai, Lin Gong, Jia-Nan Lan, Ping Dowell, Alexander C. Taylor, Graham S. Strasser, Andreas Kelly, Gemma L.
Copyright Year	2020
Description	Dysregulated expression of BCL-2 family proteins allows cancer cells to escape apoptosis. To counter this, BH3-mimetic drugs that target and inhibit select BCL-2 prosurvival proteins to induce apoptosis have been developed for cancer therapy. Venetoclax, which targets BCL-2, has been effective as therapy for patients with chronic lymphocytic leukemia, and MCL-1-targeting BH3-mimetic drugs have been extensively evaluated in preclinical studies for a range of blood cancers. Recently, BCL-W, a relatively understudied prosurvival member of the BCL-2 protein family, has been reported to be abnormally upregulated in Burkitt lymphoma (BL), diffuse large B-cell lymphoma (DLBCL), and Hodgkin lymphoma patient samples. Therefore, to determine if BCL-W would be a promising therapeutic target for B-cell lymphomas, we have examined the role of BCL-W in the sustained growth of human BL- and DLBCL-derived cell lines. We found that CRISPR/CAS9-mediated loss or short hairpin RNA-mediated knockdown of BCL-W expression in selected BL and DLBCL cell lines did not lead to spontaneous apoptosis and had no effect on their sensitivity to a range of BH3-mimetic drugs targeting other BCL-2 prosurvival proteins. Our results suggest that BCL-W is not universally required for the sustained growth and survival of human BL and DLBCL cell lines. Thus, targeting BCL-W in this subset of B-cell lymphomas may not be of broad therapeutic benefit.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6988404/pdf
Ending Page	366
Page Count	11
Starting Page	356
DOI	10.1182/bloodadvances.2019000541
Journal	Blood advances
Issue Number	2
Volume Number	4
Language	English
Publisher	American Society of Hematology
Publisher Date	2020-01-27
Access Restriction	Open
Subject Keyword	Hematology Apoptosis Lymphoma Bh3 Prosurvival Proteins Mimetic Drugs Targeting Journal: Blood advances (Vol- 41, Issue- 2)
Content Type	Text
Resource Type	Article

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