Loading...
Please wait, while we are loading the content...
Similar Documents
The Related Adhesion Focal Tyrosine Kinase (RAFTK) Is Tyrosine Phosphorylated and Participates in Colony-Stimulating Factor-1/Macrophage Colony-Stimulating Factor Signaling in Monocyte-Macrophages
| Content Provider | Scilit |
|---|---|
| Author | Hatch, William C. Ganju, Ramesh K. Hiregowdara, Dananagoud Avraham, Shalom Groopman, Jerome E. |
| Copyright Year | 1998 |
| Description | RAFTK, a novel nonreceptor protein kinase, has been shown to be involved in focal adhesion signal transduction pathways in neuronal PC12 cells, megakaryocytes, platelets, and T cells. Because focal adhesions may modulate cytoskeletal functions and thereby alter phagocytosis, cell migration, and adhesion in monocyte-macrophages, we investigated the role of RAFTK signaling in these cells. RAFTK was abundantly expressed in THP1 monocytic cells as well as in primary alveolar and peripheral blood-derived macrophages. Colony-stimulating factor-1 (CSF-1)/macrophage colony-stimulating factor (M-CSF) stimulation of THP1 cells increased the tyrosine phosphorylation of RAFTK; similar increases in phosphorylation were also detected after lipopolysaccharide stimulation. RAFTK was phosphorylated with similar kinetics in THP1 cells and peripheral blood-derived macrophages. Immunoprecipitation analysis showed associations between RAFTK and the signaling molecule phosphatidylinositol-3 (PI-3) kinase. PI-3 kinase enzyme activity also coprecipitated with the RAFTK antibody, further confirming this association. The CSF-1/M-CSF receptor c-fms and RAFTK appeared to associate in response to CSF-1/M-CSF treatment of THP1 cells. Inhibition of RAFTK by a dominant-negative kinase mutant reduced CSF-1/M-CSF–induced MAPK activity. These data indicate that RAFTK participates in signal transduction pathways mediated by CSF-1/M-CSF, a cytokine that regulates monocyte-macrophage growth and function. |
| Related Links | https://ashpublications.org/blood/article-pdf/91/10/3967/238253/3967.pdf |
| Ending Page | 3973 |
| Page Count | 7 |
| Starting Page | 3967 |
| DOI | 10.1182/blood.v91.10.3967.3967_3967_3973 |
| Journal | Blood |
| Issue Number | 10 |
| Volume Number | 91 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1998-05-15 |
| Access Restriction | Open |
| Subject Keyword | Immunology Pathology Phosphorylation Macrophages Functions Signal Transduction Pathways Stimulating Raftk Tyrosine Phosphorylated Journal: Blood (Vol- 113, Issue- 10) |
| Content Type | Text |
| Resource Type | Article |
