NDLI: Immunologic characterization of the tumor-specific bcr-abl junction in Philadelphia chromosome-positive acute lymphoblastic leukemia

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Immunologic characterization of the tumor-specific bcr-abl junction in Philadelphia chromosome-positive acute lymphoblastic leukemia

Content Provider	Scilit
Author	Denderen, J. Van Plas, D. Van Der Meeuwsen, T. Zegers, N. Boersma, W. Grosveld, G. Ewijk, W. Van
Copyright Year	1990
Description	Philadelphia (Ph′)-positive acute lymphoblastic leukemia (ALL) is highly associated with two forms of chimeric bcr-abl proteins: P190bcr- abl and P210bcr-abl. Whereas P210bcr-abl also occurs in chronic myeloid leukemia, P190bcr-abl is uniquely expressed in Ph′-positive ALL. As a consequence, P190bcr-abl is preeminently a tumor-specific marker in leukemic cells of ALL patients. Because P190bcr-abl is composed of the normal bcr and abl proteins, the major part of the P190bcr-abl molecule comprises nontumor-specific determinants. The joining region between bcr and abl, newly generated during the Ph′ translocation, is exclusively a tumor-specific epitope on the P190bcr-abl molecule. Therefore, only antibodies against the bcr-abl joining region will detect the tumor-specificity of P190bcr-abl. In this study a polyclonal antiserum, termed BP-ALL, was raised against a synthetic peptide corresponding to the bcr-abl junction in P190bcr-abl. The reactivity of BP-ALL with native P190bcr-abl derived from a Ph′-positive ALL cell line (TOM-1) was tested using immunoprecipitation analysis. BP-ALL reacted highly specifically with P190bcr-abl but not with P210bcr-abl isolated from chronic myeloid leukemia cell lines. Peptide inhibition studies further confirmed the fine specificity of BP-ALL. Our data indicate that the tumor-specific bcr-abl junction domain is exposed in an antigenic fashion on the P190bcr-abl molecule.
Related Links	https://ashpublications.org/blood/article-pdf/76/1/136/219345/136.pdf
Ending Page	141
Page Count	6
Starting Page	136
DOI	10.1182/blood.v76.1.136.bloodjournal761136
Journal	Blood
Issue Number	1
Volume Number	76
Language	English
Publisher	American Society of Hematology
Publisher Date	1990-07-01
Access Restriction	Open
Subject Keyword	Hematology Chronic Myeloid Leukemia Tumor Specific P210bcr Abl Phâ Journal: Blood (Vol- 5, Issue- 1)
Content Type	Text
Resource Type	Article

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