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Nucleotide sequence diversity of hypervariable region 1 of hepatitis C virus in Japanese hemophiliacs with chronic hepatitis C and patients with chronic posttransfusion hepatitis C
| Content Provider | Scilit |
|---|---|
| Author | Toyoda, H. Fukuda, Y. Koyama, Y. Nakano, I. Kinoshita, M. Hadama, T. Takamatsu, J. Hayakawa, T. |
| Copyright Year | 1996 |
| Description | Hemophiliac patients with chronic hepatitis C might be exposed to and become infected with multiple hepatitis C virus (HCV) strains by means of frequent use of blood products, even if they are infected with a single subtype of HCV. To test this hypothesis, we analyzed the genetic diversity of hypervariable region 1 (HVR1) of HCV in chronically infected hemophiliacs and in patients with chronic posttransfusion hepatitis with a single HCV inoculation. The diversity of nucleotide sequences in HVR1 of serum HCV RNA was compared between 21 hemophiliacs infected with a single HCV subtype and 16 patients with posttransfusion HCV infection. The number of HCV quasispecies was determined by fluorescence single-strand conformation polymorphism (SSCP) analysis. Direct sequencing was performed to determine the diversity in HVR1. The number of HCV quasispecies in the blood was 5.2 +/- 2.0 clones in hemophiliacs and 4.0 +/- 2.3 clones in posttransfusion patients, a nonsignificant difference (P = .0943). The number of sites at which the nucleotide was not homogenous in all quasispecies was significantly higher in hemophiliacs (13.0% +/- 7.4%) than in posttransfusion hepatitis patients (2.7% +/- 2.8%; P < .0001). In conclusion, there was a high degree of genetic variation in HVR1 of HCV specimens isolated from hemophiliacs compared with posttransfusion patients. These findings indicate the possibility that multiple infections of a single HCV subtype may occur among patients frequently exposed to blood products; single HCV subtypes may therefore derive from multiple origins. |
| Related Links | https://ashpublications.org/blood/article-pdf/88/4/1488/621366/1488.pdf |
| Ending Page | 1493 |
| Page Count | 6 |
| Starting Page | 1488 |
| DOI | 10.1182/blood.v88.4.1488.bloodjournal8841488 |
| Journal | Blood |
| Issue Number | 4 |
| Volume Number | 88 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1996-08-15 |
| Access Restriction | Open |
| Subject Keyword | Virology Diversity Hepatitis C Virus Nucleotide Hemophiliacs Posttransfusion Hepatitis Hcv Subtype Single Hcv Journal: Blood (Vol- 77, Issue- 4) |
| Content Type | Text |
| Resource Type | Article |
