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Interferon-alpha stimulates production of interleukin-10 in activated CD4+ T cells and monocytes
| Content Provider | Scilit |
|---|---|
| Author | Aman, Mj Tretter, T. Eisenbeis, I. Bug, G. Decker, T. Aulitzky, We Tilg, H. Huber, C. Peschel, C. |
| Copyright Year | 1996 |
| Description | In the present study, we investigated the effect of interferon-alpha (IFN-alpha) on the expression of interleukin-10 (IL-10) mRNA and protein synthesis in human monocytes and CD4+ T cells. In mononuclear cells, IFN-alpha induced expression of IL-10 mRNA and further enhanced lipopolysaccharide (LPS)-stimulated IL-10 expression. In purified monocytes, a strong expression of IL-10 mRNA induced by LPS was not further enhanced by IFN-alpha. In highly purified CD4+ T cells, IFN- alpha upregulated IL-10 mRNA upon activation with phytohemagglutinin and phorbol myristate acetate. In purified monocytes, an effect of IFN- alpha on IL-10 protein synthesis was dependent on costimulation with LPS. Maximal stimulation of IL-10 protein by IFN-alpha was seen after prolonged incubation periods of 48 to 96 hours, whereas IFN-gamma reduced IL-10 production in the early incubation period. Similar effects of IFN-alpha were observed in CD4+ T cells activated with CD3 and CD28 monoclonal antibodies. Addition of IFN-alpha caused an increase of IL-10 in culture supernatants of activated T-helper cells of more than 100% after 96 hours of incubation. In contrast, other cytokines, including IFN-gamma and IL-4, had no influence on IL-10 secretion stimulated by CD3 and CD28 in CD4+ T cells. In serum samples of IFN-alpha-treated individuals, we failed to detect an influence of cytokine treatment on IL-10 serum levels, confirming the requirement of additional activating signals for IFN-alpha-mediated effects on IL-10 synthesis. In conclusion, IFN-alpha enhances the late induction of IL- 10, which physiologically occurs upon stimulation of monocytes and T cells. Biologically, this effect might enhance the negative-feedback mechanism ascribed to IL-10, which limits inflammatory reactions. |
| Related Links | https://ashpublications.org/blood/article-pdf/87/11/4731/620166/4731.pdf |
| Ending Page | 4736 |
| Page Count | 6 |
| Starting Page | 4731 |
| DOI | 10.1182/blood.v87.11.4731.bloodjournal87114731 |
| Journal | Blood |
| Issue Number | 11 |
| Volume Number | 87 |
| Language | English |
| Publisher | American Society of Hematology |
| Publisher Date | 1996-06-01 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Protein Treatment Monocytes Antibodies Cd4 Stimulated Purified Ifn Alpha Journal: Blood (Vol- 116, Issue- 11) |
| Content Type | Text |
| Resource Type | Article |
