NDLI: DNA Microarray Gene Expression Profile of Marginal Zone versus Follicular B Cells and Idiotype Positive Marginal Zone B Cells before and after Immunization with Streptococcus pneumoniae

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DNA Microarray Gene Expression Profile of Marginal Zone versus Follicular B Cells and Idiotype Positive Marginal Zone B Cells before and after Immunization with Streptococcus pneumoniae

Content Provider	Scilit
Author	Kin, Nicholas W. Crawford, Dianna M. Liu, Jiabin Behrens, Timothy W. Kearney, John F.
Copyright Year	2008
Description	Marginal zone (MZ) B cells play an important role in the clearance of blood-borne bacterial infections via rapid T-independent IgM responses. We have previously demonstrated that MZ B cells respond rapidly and robustly to bacterial particulates. To determine the MZ-specific genes that are expressed to allow for this response, MZ and follicular (FO) B cells were sort purified and analyzed via DNA microarray analysis. We identified 181 genes that were significantly different between the two B cell populations. Ninety-nine genes were more highly expressed in MZ B cells while 82 genes were more highly expressed in FO B cells. To further understand the molecular mechanisms by which MZ B cells respond so rapidly to bacterial challenge, Id-positive and -negative MZ B cells were sort purified before (0 h) or after (1 h) i.v. immunization with heat-killed Streptococcus pneumoniae, R36A, and analyzed via DNA microarray analysis. We identified genes specifically up-regulated or down-regulated at 1 h following immunization in the Id-positive MZ B cells. These results give insight into the gene expression pattern in resting MZ vs FO B cells and the specific regulation of gene expression in Ag-specific MZ B cells following interaction with Ag.
Ending Page	6674
Page Count	12
Starting Page	6663
ISSN	00221767
e-ISSN	15506606
DOI	10.4049/jimmunol.180.10.6663
Alternate Webpage(s)	http://www.jimmunol.org/content/180/10/6663.full.pdf
Journal	The Journal of Immunology
Issue Number	10
Volume Number	180
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2008-05-15
Access Restriction	Open
Subject Keyword	Mz B Cell Fo B Cell
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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