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B7-1/2 (CD80/CD86) Direct Signaling to B Cells Enhances IgG Secretion
| Content Provider | Scilit |
|---|---|
| Author | Rau, Friederike C. Dieter, Jacquelyn Luo, Zhang Priest, Stephen O. Baumgarth, Nicole |
| Copyright Year | 2009 |
| Description | B cell responses are regulated by Ag recognition, costimulatory signals provided by interaction with helper T cells, and by innate signals. We recently provided evidence for a link between the effects of innate and costimulatory signals on B cells during influenza virus infection, by demonstrating that most B cells in the regional lymph nodes of the respiratory tract enhance surface expression of the costimulator B7-2 (CD86) within 24–48 h following infection via a type I IFNR-dependent mechanisms, a finding we are confirming here. While the role of B7-1/2 for helper T cell activation is well documented, its role in direct B cell regulation is poorly understood. Here, our in vivo studies with mixed bone marrow irradiation chimeric mice, lacking B7-1/2 only on B cells, demonstrated that B7-1/2 expression is crucial for induction of maximal local, but to a lesser extent systemic, IgG Ab responses following influenza virus infection. In contrast to mice that completely lack B7-1/2 expression, loss of B7-1/2 on B cells alone did not significantly affect germinal center formation or the extent of $CD4^{+}$ T cell activation and IFN-γ secretion. Instead, our in vitro studies identify a dramatic effect of B7-2 engagement on IgG, but not IgM secretion by already class-switched B cells. Concomitantly, B7-2 engagement induced expression of X-box binding protein 1 (XBP-1) and spliced XBP1, evidence for increased protein synthesis by these cells. Taken together, these results identify direct signaling through B7-1/2 as a potent regulator of IgG secretion by previously activated B cells. |
| Ending Page | 7671 |
| Page Count | 11 |
| Starting Page | 7661 |
| ISSN | 00221767 |
| e-ISSN | 15506606 |
| DOI | 10.4049/jimmunol.0803783 |
| Alternate Webpage(s) | http://www.jimmunol.org/content/183/12/7661.full.pdf |
| Journal | The Journal of Immunology |
| Issue Number | 12 |
| Volume Number | 183 |
| Language | English |
| Publisher | The American Association of Immunologists |
| Publisher Date | 2009-12-15 |
| Access Restriction | Open |
| Subject Keyword | Bone Marrow Cell Activation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
