NDLI: Analysis of Autoreactive CD4 T Cells in Experimental Autoimmune Encephalomyelitis after Primary and Secondary Challenge Using MHC Class II Tetramers

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Analysis of Autoreactive CD4 T Cells in Experimental Autoimmune Encephalomyelitis after Primary and Secondary Challenge Using MHC Class II Tetramers

Content Provider	Scilit
Author	Bischof, Felix Hofmann, Matthias Schumacher, Ton N. M. Vyth-Dreese, Florry A. Weissert, Robert Schild, Hansjörg Kruisbeek, Ada M. Melms, Arthur
Copyright Year	2004
Description	Experimental autoimmune encephalomyelitis (EAE), an animal model of multiple sclerosis, is primarily mediated by CD4 T cells specific for Ags in the CNS. Using MHC class II tetramers, we assessed expansion and phenotypic differentiation of polyclonal self-reactive CD4 T cells during EAE after primary and secondary challenge with the specific Ag. After EAE induction in SJL mice with proteolipid protein 139–151, CNS-specific T cells up-regulated activation markers and expanded in the draining lymph nodes and in the spleen. Less than 20% of total autoreactive T cells entered the CNS simultaneously with Th cells of other specificities. Almost all tetramer-positive cells in the CNS were activated and phenotypically distinct from the large peripheral pool. When EAE was induced in Ag-experienced mice, disease symptoms developed earlier and persisted longer; autoreactive T cells were more rapidly activated and invaded the CNS earlier. In striking contrast to specific CTLs that respond after secondary viral challenge, the absolute numbers of autoreactive CD4 T cells were not increased, indicating that the accelerated autoreactivity in Ag-experienced mice is not related to higher frequencies of autoreactive CD4 T cells.
Ending Page	2884
Page Count	7
Starting Page	2878
ISSN	00221767
e-ISSN	15506606
DOI	10.4049/jimmunol.172.5.2878
Journal	The Journal of Immunology
Issue Number	5
Volume Number	172
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2004-03-01
Access Restriction	Open
Subject Keyword	Cd4 T Cells
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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