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In Vivo Behavior of Peptide-Specific T Cells During Mucosal Tolerance Induction: Antigen Introduced Through the Mucosa of the Conjunctiva Elicits Prolonged Antigen-Specific T Cell Priming Followed by Anergy
| Content Provider | Scilit |
|---|---|
| Author | Egan, Rita M. Yorkey, Chris Black, Richard Loh, Wai Khan Stevens, Julia L. Storozynsky, Eugene Lord, Edith M. Frelinger, John G. Woodward, Jerold G. |
| Copyright Year | 2000 |
| Description | The mucosa of the conjunctiva is an important site of entry for environmental Ags as well as Ags emanating from the eye itself. However, very little is known about T cell recognition of Ag introduced through this important mucosal site. We have characterized the in vivo process of CD4 T cell recognition of Ag delivered via the conjunctival mucosa. Application of soluble OVA to the conjunctiva of BALB/c mice induced potent T cell tolerance. APC-presenting OVA peptide in vivo was only found in the submandibular lymph node and not in other lymph nodes, spleen, or nasal-associated lymphoid tissue. Similarly, in TCR transgenic DO11.10 adoptive transfer mice, OVA-specific $CD4^{+}$ T cell clonal expansion was only observed in the submandibular lymph node following conjunctival application of peptide. These experiments thus define a highly specific lymphatic drainage pathway from the conjunctiva. OVA-specific T cell clonal expansion peaked at day 3 following initiation of daily OVA administration and gradually declined during the 10-day treatment period, but remained elevated compared with nontreated adoptive transfer mice. During this period, the T cells expressed activation markers, and proliferated and secreted IL-2 in vitro in response to OVA stimulation. In contrast, these cells were unable to clonally expand in vivo, or proliferate in vitro following a subsequent OVA/CFA immunization. These results suggest that Ag applied to a mucosal site can be efficiently presented in a local draining lymph node, resulting in initial T cell priming and clonal expansion, followed by T cell anergy. |
| Ending Page | 4550 |
| Page Count | 8 |
| Starting Page | 4543 |
| ISSN | 00221767 |
| e-ISSN | 15506606 |
| DOI | 10.4049/jimmunol.164.9.4543 |
| Journal | The Journal of Immunology |
| Issue Number | 9 |
| Volume Number | 164 |
| Language | English |
| Publisher | The American Association of Immunologists |
| Publisher Date | 2000-05-01 |
| Access Restriction | Open |
| Subject Keyword | Mucosal Site Cell Priming |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
