Distinct CpG DNA and Polyinosinic-Polycytidylic Acid Double-Stranded RNA, Respectively, Stimulate CD11c− Type 2 Dendritic Cell Precursors and CD11c+ Dendritic Cells to Produce Type I IFN

Content Provider	Scilit
Author	Kadowaki, Norimitsu Antonenko, Svetlana Liu, Yong-Jun
Copyright Year	2001
Description	Two classes of nucleic acids, bacterial DNA containing unmethylated CpG motifs and dsRNA in viruses, induce the production of type I IFN that contributes to the immunostimulatory effects of these microbial molecules. Thus, it is important to determine which cells produce type I IFN in response to CpG DNA and dsRNA. $CD4^{+}CD11c^{−}$ type 2 dendritic cell precursors (pre-DC2) were identified as the main producers of type I IFN in human blood in response to viruses. Here we asked whether pre-DC2 also produce type I IFN in response to CpG DNA and dsRNA. Oligodeoxynucleotides containing particular palindromic CpG motifs induced pre-DC2, but not $CD11c^{+}$ blood DC or monocytes, to produce IFN-α. In contrast, a synthetic dsRNA, polyinosinic polycytidylic-acid, induced $CD11c^{+}$ DC, but not pre-DC2 or monocytes, to produce IFN-αβ. These data indicate that CpG DNA and polyinosinic-polycytidylic acid stimulate different types of cells to produce type I IFN and that it is important to select oligodeoxynucleotides containing particular CpG motifs to induce pre-DC2 to produce type I IFN, which may play a key role in the strong adjuvant effects of CpG DNA.
Ending Page	2295
Page Count	5
Starting Page	2291
ISSN	00221767
e-ISSN	15506606
DOI	10.4049/jimmunol.166.4.2291
Journal	The Journal of Immunology
Issue Number	4
Volume Number	166
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2001-02-15
Access Restriction	Open
Subject Keyword	Dna and Polyinosinic Produce Type Type I Ifn Polyinosinic Polycytidylic
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology