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Macrophages, CD4+ or CD8+ Cells Are Each Sufficient for Protection against Chlamydia pneumoniae Infection through their Ability to Secrete IFN-γ
| Content Provider | Scilit |
|---|---|
| Author | Rothfuchs, Antonio Gigliotti Kreuger, Maria Regina Wigzell, Hans Rottenberg, Martin E. |
| Copyright Year | 2004 |
| Description | By using a T, B, or NK cell-deficient mouse strain (recombinase-activating gene (RAG)-1−/−/common cytokine receptor γ-chain (γCR)), and T and B cell and IFN-γ-deficient (RAG-1−/−/IFN-γ−/−) mice, we have studied the generation of immunity against infection by Chlamydia pneumoniae. We found that IFN-γ secreted by innate-cell populations protect against C. pneumoniae infection. However, NK cells were not needed for such IFN-γ-dependent innate immune protection. Inoculation of wild type, but not IFN-γ−/− bone marrow-derived macrophages protected RAG-1−/−/IFN-γ−/− mice against C. pneumoniae infection. In line, pulmonary macrophages from RAG-1−/− C. pneumoniae-infected mice expressed IFN-γ mRNA. Reconstitution of RAG-1−/−/γcR−/− or RAG-1−/−/IFN-γ−/− mice with CD4+ or CD8+ cells by i.v. transfer of FACS sorted wild type spleen cells (SC) increased resistance to C. pneumoniae infection. On the contrary, no protection was observed upon transfer of IFN-γ−/− CD4+ or IFN-γ−/− CD8+ SC. T cell-dependent protection against C. pneumoniae was weaker when IFN-γR−/− CD4+ or IFN-γR−/− CD8+ SC were inoculated into RAG-1−/−/IFN-γ−/− mice. Thus both nonlymphoid and T cell-derived IFN-γ can play a central and complementary role in protection against C. pneumoniae. IFN-γ secreted by nonlymphoid cells was not required for T cell-mediated protection against C. pneumoniae; however, IFN-γ regulated T cell protective functions. |
| Ending Page | 2415 |
| Page Count | 9 |
| Starting Page | 2407 |
| ISSN | 00221767 |
| e-ISSN | 15506606 |
| DOI | 10.4049/jimmunol.172.4.2407 |
| Journal | The Journal of Immunology |
| Issue Number | 4 |
| Volume Number | 172 |
| Language | English |
| Publisher | The American Association of Immunologists |
| Publisher Date | 2004-02-15 |
| Access Restriction | Open |
| Subject Keyword | Innate Immune Bone Marrow Ifn Γ |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
