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Human Gut Commensal Membrane Vesicles Modulate Inflammation by Generating M2-like Macrophages and Myeloid-Derived Suppressor Cells
| Content Provider | Scilit |
|---|---|
| Author | Bulut, Esin Alpdundar Kocabas, Banu Bayyurt Yazar, Volkan Aykut, Gamze Guler, Ulku Salih, Bekir Yilmaz, Naz Surucu Ayanoglu, Ihsan Cihan Polat, Muammer Merve Akcali, Kamil Can Gursel, Ihsan Gursel, Mayda |
| Copyright Year | 2020 |
| Description | Immunomodulatory commensal bacteria modify host immunity through delivery of regulatory microbial-derived products to host cells. Extracellular membrane vesicles (MVs) secreted from symbiont commensals represent one such transport mechanism. How MVs exert their anti-inflammatory effects or whether their tolerance-inducing potential can be used for therapeutic purposes remains poorly defined. In this study, we show that MVs isolated from the human lactic acid commensal bacteria Pediococcus pentosaceus suppressed Ag-specific humoral and cellular responses. MV treatment of bone marrow–derived macrophages and bone marrow progenitors promoted M2-like macrophage polarization and myeloid-derived suppressor cell differentiation, respectively, most likely in a TLR2-dependent manner. Consistent with their immunomodulatory activity, MV-differentiated cells upregulated expression of IL-10, arginase-1, and PD-L1 and suppressed the proliferation of activated T cells. MVs' anti-inflammatory effects were further tested in acute inflammation models in mice. In carbon tetrachloride–induced fibrosis and zymosan-induced peritonitis models, MVs ameliorated inflammation. In the dextran sodium sulfate–induced acute colitis model, systemic treatment with MVs prevented colon shortening and loss of crypt architecture. In an excisional wound healing model, i.p. MV administration accelerated wound closure through recruitment of PD-L1–expressing myeloid cells to the wound site. Collectively, these results indicate that P. pentosaceus–derived MVs hold promise as therapeutic agents in management/treatment of inflammatory conditions. |
| Ending Page | 2718 |
| Page Count | 12 |
| Starting Page | 2707 |
| ISSN | 00221767 |
| e-ISSN | 15506606 |
| DOI | 10.4049/jimmunol.2000731 |
| Journal | The Journal of Immunology |
| Issue Number | 10 |
| Volume Number | 205 |
| Language | English |
| Publisher | The American Association of Immunologists |
| Publisher Date | 2020-11-15 |
| Access Restriction | Open |
| Subject Keyword | Bone Marrow Pd L1 M2 Like Suppressor Cell Myeloid Derived Derived Suppressor Like Macrophages Inflammatory Effects |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
