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Invariant or Highly Conserved TCR α Are Expressed on Double-Negative (CD3+CD4−CD8−) and CD8+ T Cells
| Content Provider | Scilit |
|---|---|
| Author | Han, Mei Harrison, Lisa Kehn, Patricia Stevenson, Kimberly Currier, Jeffrey Robinson, Mary Ann |
| Copyright Year | 1999 |
| Description | TCR α (TCRA) expression was examined in RNA samples from PBMC and isolated populations of $CD4^{+}$, $CD8^{+}$, and DN T cells from 15 healthy individuals. The expressed TCR repertoire was surveyed using spectratype analysis, a technique that displays the distribution of complementarity determining region 3 (CDR3) lengths for each TCRAV gene family. The results revealed the presence of unusual populations of double-negative (DN; $CD4^{−}CD8^{−}CD3^{+}$) T cells that express invariant or conserved TCRAV4A, AV7, AV19, and AV24 chains. Each of the conserved TCRA families was over-represented in >70% of the individuals studied, and all individuals expressed at least one of the over-represented TCRAV families. Over-represented conserved AV4A or AV7 sequences were also present in $CD8^{+}$ T cells from most donors. The extent of TCRA sequence conservation is unparalleled. TCRAV4A, AV19, and AV24 sequences were invariant, although AV4A and AV19 transcripts contained N region additions. TCRAV24 transcripts derived from the direct juxtaposition of V and J gene segments. TCRAV7 sequences showed some diversity in two amino acids encoded at junctions of V and J gene segments. Although derivation of DN T cells with conserved TCRA chains is puzzling, the wide-spread expression of these unusual cells suggests an important function. |
| Ending Page | 311 |
| Page Count | 11 |
| Starting Page | 301 |
| ISSN | 00221767 |
| e-ISSN | 15506606 |
| DOI | 10.4049/jimmunol.163.1.301 |
| Journal | The Journal of Immunology |
| Issue Number | 1 |
| Volume Number | 163 |
| Language | English |
| Publisher | The American Association of Immunologists |
| Publisher Date | 1999-07-01 |
| Access Restriction | Open |
| Subject Keyword | Dn T Cells |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
