NDLI: IFN-β Facilitates Neuroantigen-Dependent Induction of CD25+ FOXP3+ Regulatory T Cells That Suppress Experimental Autoimmune Encephalomyelitis

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IFN-β Facilitates Neuroantigen-Dependent Induction of CD25+ FOXP3+ Regulatory T Cells That Suppress Experimental Autoimmune Encephalomyelitis

Content Provider	Scilit
Author	Wang, Duncheng Ghosh, Debjani Islam, S. M. Touhidul Moorman, Cody D. Thomason, Ashton E. Wilkinson, Daniel S. Mannie, Mark D.
Copyright Year	2016
Abstract	This study introduces a flexible format for tolerogenic vaccination that incorporates IFN-β and neuroantigen (NAg) in the Alum adjuvant. Tolerogenic vaccination required all three components, IFN-β, NAg, and Alum, for inhibition of experimental autoimmune encephalomyelitis (EAE) and induction of tolerance. Vaccination with IFN-β + NAg in Alum ameliorated NAg-specific sensitization and inhibited EAE in C57BL/6 mice in pretreatment and therapeutic regimens. Tolerance induction was specific for the tolerogenic vaccine Ag PLP178-191 or myelin oligodendrocyte glycoprotein (MOG)35–55 in proteolipid protein– and MOG-induced models of EAE, respectively, and was abrogated by pretreatment with a depleting anti-CD25 mAb. IFN-β/Alum–based vaccination exhibited hallmarks of infectious tolerance, because IFN-β + OVA in Alum–specific vaccination inhibited EAE elicited by OVA + MOG in CFA but not EAE elicited by MOG in CFA. IFN-β + NAg in Alum vaccination elicited elevated numbers and percentages of $FOXP3^{+}$ T cells in blood and secondary lymphoid organs in 2D2 MOG-specific transgenic mice, and repeated boosters facilitated generation of activated $CD44^{high}$ $CD25^{+}$ regulatory T cell (Treg) populations. IFN-β and MOG35–55 elicited suppressive $FOXP3^{+}$ Tregs in vitro in the absence of Alum via a mechanism that was neutralized by anti–TGF-β and that resulted in the induction of an effector $CD69^{+}$ $CTLA-4^{+}$ $IFNAR^{+}$ $FOXP3^{+}$ Treg subset. In vitro IFN-β + MOG–induced Tregs inhibited EAE when transferred into actively challenged recipients. Unlike IFN-β + NAg in Alum vaccines, vaccination with TGF-β + MOG35-55 in Alum did not increase Treg percentages in vivo. Overall, this study indicates that IFN-β + NAg in Alum vaccination elicits NAg-specific, suppressive $CD25^{+}$ Tregs that inhibit CNS autoimmune disease. Thus, IFN-β has the activity spectrum that drives selective responses of suppressive $FOXP3^{+}$ Tregs.
Ending Page	3007
Page Count	16
Starting Page	2992
ISSN	00221767
e-ISSN	15506606
DOI	10.4049/jimmunol.1500411
Journal	The Journal of Immunology
Issue Number	8
Volume Number	197
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2016-10-15
Access Restriction	Open
Subject Keyword	Regulatory T Cells Interferon-beta Eae/ms
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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